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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Melanopsin and rhodopsin mediate UVA-induced immediate pigment darkening: Unravelling the photosensitive system of the skin

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Author(s):
Monteiro de Assis, Leonardo Vinicius [1] ; Moraes, Maria Nathalia [1] ; Magalhaes-Marques, Keila Karoline [1] ; de Lauro Castrucci, Ana Maria [2, 1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Biosci, Dept Physiol, Lab Comparat Physiol Pigmentat, Sao Paulo - Brazil
[2] Univ Virginia, Dept Biol, Charlottesville, VA - USA
Total Affiliations: 2
Document type: Journal article
Source: EUROPEAN JOURNAL OF CELL BIOLOGY; v. 97, n. 3, p. 150-162, APR 2018.
Web of Science Citations: 8
Abstract

The mammalian skin has a photosensitive system comprised by several opsins, including rhodopsin (OPN2) and melanopsin (OPN4). Recently, our group showed that UVA (4.4 kJ/m(2)) leads to immediate pigment darkening (IPD) in murine normal and malignant melanocytes. We show the role of OPN2 and OPN4 as UVA sensors: UVA-induced IPD was fully abolished when OPN4 was pharmacologically inhibited by AA9253 or when OPN2 and OPN4 were knocked down by siRNA in both cell lines. Our data, however, demonstrate that phospholipase C/protein kinase C pathway, a classical OPN4 pathway, is not involved in WA-induced IPD in either cell line. Nonetheless, in both cell types we have shown that: a) intracellular calcium signal is necessary for WA-induced IPD; b) the involvement of CaMK II, whose inhibition, abolished the UVA-induced IPD; c) the role of CAMK II/NOS/sGC/cGMP pathway in the process since inhibition of either NOS or sGC abolished the WA-induced IPD. Taken altogether, we show that OPN2 and OPN4 participate in IPD induced by WA in murine normal and malignant melanocytes through a conserved common pathway. Interestingly, upon knockdown of OPN2 or OPN4, the UVA-driven IPD is completely lost, which suggests that both opsins are required and cooperatively signal in murine both cell lines. The participation of OPN2 and OPN4 system in UVA radiation-induced response, if proven to take place in human skin, may represent an interesting pharmacological target for the treatment of depigmentary disorders and skin-related cancer. (AU)

FAPESP's process: 14/16412-9 - Mechanisms of clock genes thermo-modulation in peripheral tissue of mammals: role of TRP channels
Grantee:Maria Nathália de Carvalho Magalhães Moraes Figueira Borges
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/24337-4 - Clock genes modulation by UVA/blue light stimulation in normal melan-A and transformed B-16 melanocytes
Grantee:Leonardo Vinícius Monteiro de Assis
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 12/50214-4 - Biological clock setting by light and temperature: phylogenetic aspects
Grantee:Ana Maria de Lauro Castrucci
Support Opportunities: Research Projects - Thematic Grants