Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of Cephalosporins by UHPLC-DAD Using Molecularly Imprinted Polymers

Full text
Author(s):
Baeza-Fonte, Alen N. [1] ; Garces-Lobo, Idenia [2] ; Luaces-Alberto, Markel D. [2] ; Goncalves, Luis Moreira [3, 4] ; Sotomayor, Maria D. P. T. [5] ; Valdes-Gonzalez, Aristides C. [1]
Total Authors: 6
Affiliation:
[1] Univ Havana, Inst Sci & Technol Mat, Universitary Lab Characterizat Struct Subst, Calle Zapata & G S-N, Havana 10400 - Cuba
[2] Univ Havana, Fac Chem, Dept Analyt Chem, Calle Zapata & G S-N, Havana 10400 - Cuba
[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[4] Univ Porto FCUP, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE LAQV, Rua Campo Alegre S-N, P-4169007 Oporto - Portugal
[5] UNESP Univ Estadual Paulista, Inst Quim, Dept Quim Analit, Ave Prof Francisco Degni 55, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Chromatographic Science; v. 56, n. 2, p. 187-193, FEB 2018.
Web of Science Citations: 8
Abstract

Molecularly imprinted polymers (MIPs) were synthesized for the determination of cephalosporins (cephazolin, ceftazidime, cefotaxime, ceftriaxone, cefepime and cephalexin) by ultra-high performance liquid chromatography with diode-array detection (UHPLC-DAD). After optimization, MIPs were synthesized using cephazolin as the template, methacrylic acid as the functional monomer, triethylenglycol dimethacrylate as the crosslinker, acetonitrile/dimethylsulfoxide as porogens and benzoyl peroxide as the radical initiator. Not only this is a novel route of MIP synthesis for cephalosporins, but also this choice of analytes is unique. Chromatographic separation was performed in a C-8 column using a binary gradient with trifluoroacetic acid 0.1% in water and acetonitrile. Linearity was assessed up to 100 mu g mL(-1) and linear correlation coefficients (r) were all higher than 0.99, limits of detection were within the range of 3-12 ng mL(-1), and recoveries from 86 to 102% were obtained for concentrations between 0.05 and 1.0 mu g mL(-1). (AU)

FAPESP's process: 16/06926-0 - Development and application of new nanostructured chemical sensors and applications of new nanostructured chemical sensors based on biomimetic polymers
Grantee:Maria Del Pilar Taboada Sotomayor
Support type: Research Grants - Visiting Researcher Grant - International