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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of Cephalosporins by UHPLC-DAD Using Molecularly Imprinted Polymers

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Author(s):
Baeza-Fonte, Alen N. [1] ; Garces-Lobo, Idenia [2] ; Luaces-Alberto, Markel D. [2] ; Goncalves, Luis Moreira [3, 4] ; Sotomayor, Maria D. P. T. [5] ; Valdes-Gonzalez, Aristides C. [1]
Total Authors: 6
Affiliation:
[1] Univ Havana, Inst Sci & Technol Mat, Universitary Lab Characterizat Struct Subst, Calle Zapata & G S-N, Havana 10400 - Cuba
[2] Univ Havana, Fac Chem, Dept Analyt Chem, Calle Zapata & G S-N, Havana 10400 - Cuba
[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[4] Univ Porto FCUP, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE LAQV, Rua Campo Alegre S-N, P-4169007 Oporto - Portugal
[5] UNESP Univ Estadual Paulista, Inst Quim, Dept Quim Analit, Ave Prof Francisco Degni 55, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Chromatographic Science; v. 56, n. 2, p. 187-193, FEB 2018.
Web of Science Citations: 8
Abstract

Molecularly imprinted polymers (MIPs) were synthesized for the determination of cephalosporins (cephazolin, ceftazidime, cefotaxime, ceftriaxone, cefepime and cephalexin) by ultra-high performance liquid chromatography with diode-array detection (UHPLC-DAD). After optimization, MIPs were synthesized using cephazolin as the template, methacrylic acid as the functional monomer, triethylenglycol dimethacrylate as the crosslinker, acetonitrile/dimethylsulfoxide as porogens and benzoyl peroxide as the radical initiator. Not only this is a novel route of MIP synthesis for cephalosporins, but also this choice of analytes is unique. Chromatographic separation was performed in a C-8 column using a binary gradient with trifluoroacetic acid 0.1% in water and acetonitrile. Linearity was assessed up to 100 mu g mL(-1) and linear correlation coefficients (r) were all higher than 0.99, limits of detection were within the range of 3-12 ng mL(-1), and recoveries from 86 to 102% were obtained for concentrations between 0.05 and 1.0 mu g mL(-1). (AU)

FAPESP's process: 16/06926-0 - Development and application of new nanostructured chemical sensors and applications of new nanostructured chemical sensors based on biomimetic polymers
Grantee:Maria Del Pilar Taboada Sotomayor
Support type: Research Grants - Visiting Researcher Grant - International