CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis

Author(s):Show less -

de Faria Junior, Geraldo M. ^{[1, 2]} ; Ayo, Christiane M. ^{[1, 2]} ; de Oliveira, Amanda P. ^[1] ; Lopes, Alessandro G. ^[3] ; Frederico, Fabio B. ^{[1, 4, 2]} ; Silveira-Carvalho, Aparecida P. ^[1] ; Previato, Mariana ^{[1, 2]} ; Barbosa, Amanda P. ^{[4, 2]} ; Murata, Fernando H. A. ^{[1, 2]} ; de Almeida Junior, Gildasio Castello ^{[4, 2]} ; Siqueira, Rubens Camargo ^[2] ; de Mattos, Luiz C. ^{[1, 2]} ; Brandao de Mattos, Cinara C. ^{[1, 2]}

Affiliation

^[1] Fac Med Sao Jose Rio Preto FAMERP, Mol Biol Dept, Immunogenet Lab, Sao Jose Do Rio Preto, SP - Brazil

^[2] Fac Med Sao Jose Rio Preto FAMERP, Toxoplasma Res Grp, Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Estadual Paulista Julio Mesquita Filho UNESP, Inst Biociencias Letras & Ciencias Exatas IBILCE, Biol Dept, Sao Jose Do Rio Preto, SP - Brazil

^[4] Hosp Base, Fundacao Fac Reg Med Sao Jose Rio Preto HP FUNFAR, Ophthalmol Outpatient Clin, Sao Jose Do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: Acta Tropica; v. 178, p. 276-280, FEB 2018.
• Web of Science Citations: 1

Abstract

C-C chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5A32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as `with ocular toxoplasmosis' (G1: n = 160) or `without ocular toxoplasmosis' (G2: n = 160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5A32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD +/- 19.3, median: 46 {[}range: 18-95]; G2: mean age: 61.3, SD +/- 13.7, median: 61 {[}range: 21-871; G3: mean age: 38.8, SD +/- 17.9, median: 34 {[}range: 18-80]) was statistically significant (G1 vs.G2: p-value < 0.0001; t = 7.21; DF = 318; G1 vs.G3: p-value < 0.0001; t = 4.32; DF = 318; G2 vs. G3: p-value < 0.0001; t = 9.62; DF = 318). The Nagelkerke r(2) value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value = 0.008; OR = 0.261), AA (p-value = 0.007; OR = 2.974) and AG genotypes (p-value = 0.018; OR = 2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue. (AU)

• FAPESP's process: 13/10050-5 - Serological, molecular and images in patients with active lesions or acute relapse of ocular infection by Toxoplasma gondii: a study follow-up
• Grantee: Mariana Previato
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 09/17540-2 - Histo blood groups like risk factor for ocular toxoplasmosis
• Grantee: Luiz Carlos de Mattos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/15879-8 - Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS)
• Grantee: Fernando Henrique Antunes Murata
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/17226-7 - Biomarkers in ocular diseases
• Grantee: Gildásio Castello de Almeida Júnior
• Support Opportunities: Regular Research Grants