Changes of bacterial communities in the rhizosphere of sugarcane under elevated concentration of atmospheric CO2

Author(s)

Da Costa, Diogo P. ^[1] ; Dias, Armando C. F. ^[1] ; Cotta, Simone R. ^[1] ; Vilela, Daniella ^[2] ; De Andrade, Pedro A. M. ^[1] ; Pellizari, Vivian H. ^[2] ; Andreote, Fernando Dini ^[1]

Affiliation

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Oceanog Inst, Lab Microbial Ecol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Global Change Biology Bioenergy; v. 10, n. 2, p. 137-145, FEB 2018.
• Web of Science Citations: 5

Abstract

It is believed that climate change will influence most of interactions that sustain life on Earth. Among these, the recruitment exerted by plants in their roots vicinity can change, leading to differential assemblages of microbiomes in the rhizosphere. We approached this issue analyzing the variations in the composition of bacterial communities in the rhizosphere of sugarcane cultivated under two concentrations of atmospheric CO2 (350 or 700 ppm). In addition to the analysis of bacterial community, the use of DNA-SIP allowed the comparison of bacterial groups assimilating roots exudates (based on C-13-labeled DNA) in both conditions, in a period of 8 days after the CO2 pulse. The separation of C-13-DNA indicated the low but increasing frequency of labeling in the rhizosphere, as averages of 0.6, 2.4 and 5.0% of total DNA were labeled after 2, 4, and 8 days after the (CO2)-C-13 pulse, respectively. Based on large-scale sequencing of the V6 region in the gene 16S rRNA, we found an increase in the bacterial diversity in the C-13-DNA along the sampling period. We also describe the occurrence of distinct bacterial groups assimilating roots exudates from sugarcane cultivated under each CO2 concentration. Bacilli, Gammaproteobacteria, and Clostridia showed high affinity for the C-sources released by sugarcane under 350 ppm of CO2, while under elevated concentration of CO2, the assimilation of roots exudates was prevalently made by members of Bacilli and Betaproteobacteria. The communities became more similar along time (4 and 8 days after CO2 pulse), in both concentrations of CO2, electing Actinobacteria, Sphingobacteriia, and Alphaproteobacteria as the major cross-feeders on sugarcane exudates. In summary, we described the bacterial groups with higher affinity to assimilate roots exudates in the rhizosphere of sugarcane, and also demonstrated that the rhizosphere community can be differentially assembled in a future scenario with increased contents of CO2. (AU)

• FAPESP's process: 11/04857-8 - Description of microbial community rhizosphere of sugar cane through the technical SIP (stable isotope probing)
• Grantee: Diogo Paes da Costa
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/50353-7 - Microbial consortia for biowaste management: life cycle analysis of novel strategies of bioconversion (MICROWASTE)
• Grantee: Fernando Dini Andreote
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 12/14534-4 - METATRANSCRIPTOMICS AND GENOMIC CONTEXT IN MICROBIAL COMMUNITIES INVOLVED IN THE BIOGEOCHEMICAL CYCLES IN MANGROVES
• Grantee: Armando Cavalcante Franco Dias
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/07343-5 - Bacterial and archaeal diversity in soils cultivated with sugarcane in the State of São Paulo: a biogeographic approach
• Grantee: Ademir Durrer Bigaton
• Support Opportunities: Scholarships in Brazil - Doctorate