Changes in Global Transcriptional Profiling of Women Following Obesity Surgery Bypass

Author(s):Show less -

de Souza Pinhel, Marcela Augusta ^[1] ; Noronha, Natalia Yumi ^[1] ; Nicoletti, Carolina Ferreira ^[1] ; Parente de Oliveira, Bruno Affonso ^[1] ; Cortes-Oliveira, Cristiana ^[1] ; Pinhanelli, Vitor Caressato ^[1] ; Salgado Junior, Wilson ^[2] ; Machry, Ana Julia ^[3] ; da Silva Junior, Wilson Araujo ^[3] ; Silva Souza, Doroteia Rossi ^[4] ; Marchini, Julio Sergio ^[1] ; Nonino, Carla Barbosa ^[1]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Lab Nutrigen Studies, FMRP, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, FMRP, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, FMRP, Ribeirao Preto, SP - Brazil

^[4] Sao Jose do Rio Preto Med Sch, Dept Mol Biol, Sao Jose Do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: OBESITY SURGERY; v. 28, n. 1, p. 176-186, JAN 2018.
• Web of Science Citations: 6

Abstract

Differential gene expression in peripheral blood mononuclear cells (PBMCs) after Roux-en-Y gastric bypass (RYGB) is poorly characterized. Markers of these processes may provide a deeper understanding of the mechanisms that underlie these events. The main goal of this study was to identify changes in PBMC gene expression in women with obesity before and 6 months after RYGB-induced weight loss. The ribonucleic acid (RNA) of PBMCs from 13 obese women was analyzed before and 6 months after RYGB; the RNA of PBMCs from nine healthy women served as control. The gene expression levels were determined by microarray analysis. Significant differences in gene expression were validated by real-time quantitative polymerase chain reaction (RT-qPCR). Microarray analysis for comparison of the pre- and postoperative periods showed that 1366 genes were differentially expressed genes (DEGs). The main pathways were related to gene transcription; lipid, energy, and glycide metabolism; inflammatory and immunological response; cell differentiation; oxidative stress regulation; response to endogenous and exogenous stimuli; substrate oxidation; mTOR signaling pathway; interferon signaling; mitogen-activated protein kinases (MAPK), cAMP response element binding protein (CREB1), heat shock factor 1 (HSF1), and sterol regulatory element binding protein 1c (SREBP-1c) gene expression; adipocyte differentiation; and methylation. Six months after bariatric surgery and significant weight loss, many molecular pathways involved in obesity and metabolic diseases change. These findings are an important tool to identify potential targets for therapeutic intervention and clinical practice of nutritional genomics in obesity. (AU)

• FAPESP's process: 13/12819-4 - Genetic, clinical, biochemical and heart rate variability changes in grade III Obesity pre and post bariatric surgery
• Grantee: Marcela Augusta de Souza Pinhel
• Support Opportunities: Scholarships in Brazil - Post-Doctoral