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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition

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Author(s):
Amorim, Fernanda Gobbi [1, 2] ; Costa, Tassia Rafaela [1] ; Baiwir, Dominique [3] ; De Pauw, Edwin [4] ; Quinton, Loic [4] ; Sampaio, Suely Vilela [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Lab Toxinol, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ave Cafe S-No Campus Univ, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Liege, GIGA Inst, GIGA Prote Facil, B-4000 Liege - Belgium
[4] Univ Liege, Dept Chem, Lab Mass Spectrometry, B-4000 Liege - Belgium
Total Affiliations: 4
Document type: Journal article
Source: TOXINS; v. 10, n. 5 MAY 2018.
Web of Science Citations: 5
Abstract

Venom composition varies across snakes from all taxonomic levels and is influenced by the snakes' age, habitat, diet, and sexual dimorphism. The present study reports the first in-depth investigation of venom composition in male and female Bothrops moojeni (B. moojeni) snakes (BmooM and BmooF, respectively) through three proteomics approaches associated with functional, cytotoxic, and immunoreactivity characterization. Compared with BmooM venom, BmooF venom exhibited weaker hyaluronidase, metalloproteinase, and phospholipase activity; stronger recognition by anti-bothropic serum; 1.4-fold stronger cytotoxicity; and greater number of peptides. The increased L-amino acid oxidase expression probably accounted for the stronger immunoreactivity and cytotoxicity of BmooF venom. BmooF and BmooM venom shared only 19% peptides. Some venom components were gender-specific, such as phospholipases B, phospholipase inhibitor, and hyaluronidases in BmooM, and cysteine-rich secretory proteins in BmooF. In conclusion, we describe herein the first proteomics study of B. moojeni snake venom and an in-depth characterization of gender-specific differences in venom composition. Altogether, our findings not only stress the importance of considering the snake's gender during antivenom production, but also help to identify new potential drugs and biotechnological tools. (AU)

FAPESP's process: 16/20641-9 - Integration of omics approaches to profile the venom of the Bothrops moojeni snake: unreavelling new toxins and ontogenic divergences
Grantee:Fernanda Gobbi Amorim
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/26609-7 - Bothrops moojeni snake venomics: application of toxins of biotechnological interest obtained by omics techniques
Grantee:Fernanda Gobbi Amorim
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/00740-0 - Therapeutic potential evaluation of L-amino acid oxidases isolated from snake venoms as antitumor: genotoxicity and gene expression studies
Grantee:Tássia Rafaella Costa
Support type: Scholarships in Brazil - Post-Doctorate