Prevalence and molecular features of ESBL/pAmpC-producing Enterobacteriaceae in healthy and diseased companion animals in Brazil

Author(s)

Melo, Luana C. ^{[1, 2]} ; Oresco, Cintia ^[1] ; Leigue, Lucianne ^[1] ; Netto, Hildebrando M. ^[3] ; Melville, Priscilla A. ^[4] ; Benites, Nilson R. ^[4] ; Saras, Estelle ^[2] ; Haenni, Marisa ^[2] ; Lincopan, Nilton ^[1] ; Madec, Jean-Yves ^[2]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[2] Univ Claude Bernard Lyon 1, Unite Antibioresistance & Virulence Bacteriennes, ANSES Lab Lyon, Lyon - France

^[3] Ctr Zoonosis Control, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Prevent Vet Med & Anim Hlth, Fac Vet Med, Sao Paulo - Brazil

• Document type: Journal article
• Source: Veterinary Microbiology; v. 221, p. 59-66, JUL 2018.
• Web of Science Citations: 4

Abstract

Extended-spectrum beta-lactamase (ESBL)-and plasmid-mediated AmpC (pAmpC)-carrying Enterobacteriaceae have widely disseminated in human, animal and environmental reservoirs. Pets have been recognized as a source of ESBL/pAmpC worldwide, and are possibly also a source of human contamination. The aim of this study was to document to what extent cats and dogs may act as a driving force in the spread of ESBLs and pAmpCs in Brazil. A total of 113 healthy stray cats and dogs and 74 sick pets were sampled, and extended-spectrum cephalosporinresistant Enterobacteriaceae (ESC-R) were detected in 28/113 (24.8%) and 8/74 (10.8%) tested animals, respectively. Different Enterobacteriaceae isolates (mostly E. coli), a large number of E. coli clones (with ST90, ST457, ST973 and ST2541 being predominant), and several ESBL/pAmpC genes and plasmids were characterized, highlighting the ability of stray and pet cats and dogs to further spread a wide range of ESC-resistance determinants. The ESBL phenotype was due to the bla(CTX-M-2) and bla(CTX-M-8) genes, as found in human epidemiology in Brazil, but bla(CTX.M.9) and bla(CTX-M-15) were also identified. The pAmpC phenotype was systematically due to the presence of the bla(CMY-2) gene, mostly carried by Incl1 ST12 plasmids. Our results showed that pets can be considered a significant reservoir of multidrug-resistant bacteria in Brazil. This is especially true for healthy stray dogs that displayed the highest prevalence (24.8%) of ESBLs/pAmpC resistance determinants, which can then be further spread both to the environment and to other animals or humans by contact. (AU)

• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/04725-0 - Monitoring of multidrug-resistant Gram-negative bacteria of medical importance (human & veterinary): clinical/environmental impact and development of therapeutic alternatives and technology innovation products
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants