Biological and Chemical Control of Sclerotinia sclerotiorum using Stachybotrys levispora and Its Secondary Metabolite Griseofulvin

Author(s):Show less -

Ribeiro, Alany Ingrid ^[1] ; Costa, Eveline Soares ^[1] ; Thomasi, Sergio Scherrer ^[2] ; Ribeiro Brandao, Dayson Fernando ^[3] ; Vieira, Paulo Cesar ^[1] ; Fernandes, Joao Batista ^[1] ; Forim, Moacir Rossi ^[1] ; Ferreira, Antonio Gilberto ^[1] ; Pascholati, Sergio Florentino ^[3] ; Pascholati Gusmao, Luis Fernando ^[4] ; das Gracas Fernandes da Silva, Maria Fatima ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Quim, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Lavras, Dept Quim, CP 3037, BR-37200000 Lavras - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[4] Univ Estadual Feira de Santana, Dept Ciencias Biol, BR 116, Km 03, BR-44031460 Feira De Santana, BA - Brazil

• Document type: Journal article
• Source: Journal of Agricultural and Food Chemistry; v. 66, n. 29, p. 7627-7632, JUL 25 2018.
• Web of Science Citations: 1

Abstract

Sclerotinia sclerotiorum is responsible for the white mold of soybeans, and the difficulty to control the disease in Brazil is causing million-dollar damages. Stachybotrys levispora has shown activity against S. sclerotiorum. In our present investigation, we analyzed the chemical basis of this inhibition. Eight compounds were isolated, and using spectroscopic methods, we identified their structures as the known substances 7-dechlorogriseofulvin, 7-dechlorodehydrogriseofulvin, griseofulvin, dehydrogriseofulvin, 3,13-dihydroxy-5,9,11-trimethoxy-1-methylbenzophenone, griseophenone A, 13-hydroxy-3,5,9,11-tetramethoxy-1-methylbenzophenone, and 12-chloro-13-hydroxy-3,5,9,11-tetramethoxy-1-methylbenzophenone. Griseofulvin inhibited the mycelial growth of S. sclerotiorum at 2 mu g mL(-1). Thus, the antagonistic effect of S. levispora to S. sclerotiorum may well be due to the presence of griseofulvins. Our results stimulate new work on the biosynthesis of griseofulvins, to locate genes that encode key enzymes in these routes and use them to increase the production of these compounds and thus potentiate the fungicide effect of this fungus. S. levispora represents an agent for biocontrol, and griseofulvin represents a fungicide to S. sclerotiorum. (AU)

• FAPESP's process: 14/50918-7 - INCT 2014: for the Biorational Control of Pest-Insect and Phytopathogens
• Grantee: Maria Fátima das Graças Fernandes da Silva
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/16117-2 - CHEMICAL STUDY OF PLANTS, FUNGI AND BACTERIA: A CONTRIBUTION TO BIORATIONAL CONTROL OF CROP PESTS
• Grantee: Maria Fátima das Graças Fernandes da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/25299-6 - Integrated studies for leaf cutting control
• Grantee: João Batista Fernandes
• Support Opportunities: Research Projects - Thematic Grants