Molecular diagnosis of Strongyloides stercoralis among transplant candidates

Author(s):Show less -

Paula, Fabiana M. ^{[1, 2]} ; Malta, Fernanda M. ^{[1, 3]} ; Marques, Priscilla D. ^{[1, 2]} ; Melo, Gessica B. ^{[1, 2]} ; Corral, Marcelo A. ^[2] ; Gottardi, Maiara ^[2] ; Pinho, Joao R. R. ^{[1, 3]} ; Goncalves, Elenice M. N. ^[4] ; Castilho, Vera L. P. ^[4] ; Pierrotti, Ligia C. ^[5] ; Abdala, Edson ^[5] ; Costa, Silvia F. ^[5] ; Chieffi, Pedro P. ^[6] ; Gryschek, Ronaldo C. B. ^{[5, 1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Fac Med, Lab Invest Med, LIM Lab Imunopatol Esquistossomose 06, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Hosp Clin, Fac Med, Lab Invest Med, LIM Lab Gastroenterol & Hepatol Trop 07, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Hosp Clin, Fac Med, Div Lab Cent, Secao Parasitol, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med, Sao Paulo - Brazil

^[6] Fac Ciencias Med, Santa Casa, SP - Brazil

• Document type: Journal article
• Source: TRANSPLANT INFECTIOUS DISEASE; v. 20, n. 4 AUG 2018.
• Web of Science Citations: 0

Abstract

Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (cPCR) and real-time PCR (qPCR). Polymerase chain reaction (PCR) and real-time PCR (qPCR) targeting the 18S rRNA gene for detection of Strongyloides stercoralis infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods. S.stercoralis larvae were visualized in 15/150 (10.0%) transplant candidates by parasitological methods. DNA from S.stercoralis was amplified in 26/150 (17.3%) and 49/150 (32.7%) stool samples of transplant candidates, using cPCR and qPCR, respectively. The results suggest that molecular methods, especially qPCR, should be used as an additional tool for diagnostic of S.stercoralis infection among transplant candidates. (AU)

• FAPESP's process: 10/51110-2 - Investigation of diagnostic techniques for identification of infection S. stercoratis in patients with different forms of immunosuppression
• Grantee: Pedro Paulo Chieffi
• Support Opportunities: Regular Research Grants