Pouteria ramiflora (Mart.) Radlk. extract: Flavonoids quantification and chemopreventive effect on HepG2 cells

Author(s)

Tuttis, Katiuska ^[1] ; Gomes da Costa, Daryne Lu Maldonado ^{[2, 3]} ; Nunes, Higor Lopes ^[1] ; Leal Specian, Ana Flavia ^[1] ; Serpeloni, Juliana Mara ^[1] ; dos Santos, Lourdes Campaner ^[2] ; Varanda, Eliana Aparecida ^[4] ; Vilegas, Wagner ^[5] ; Martinez-Lopez, Wilner ^[6] ; de Syllos Colus, Ilce Mara ^[1]

Affiliation

^[1] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Biol Geral, Londrina, Parana - Brazil

^[2] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Quim, Dept Quim Organ, Araraquara, SP - Brazil

^[3] Inst Fed Educ Ciencia & Tecnol Mato Grosso IFMT, Cuiaba, Mato Grosso - Brazil

^[4] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Ciencias Farmaceut, Dept Ciencias Biol, Araraquara, SP - Brazil

^[5] Univ Estadual Paulista Julio de Mesquita Filho UN, Campus Expt Litoral Paulista, Sao Vicente, SP - Brazil

^[6] Inst Invest Biol Clemente Estable IIBCE Montevide, Montevideo - Uruguay

• Document type: Journal article
• Source: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 81, n. 16, p. 792-804, 2018.
• Web of Science Citations: 5

Abstract

Pouteria ramiflora (Mart.) Radlk., popularly known as curriola, is commonly used in Brazil as medicinal plant to treat worm infections, dysentery, pain, inflammation, hyperlipidemia, and obesity. At present the safety of this extract when used therapeutically in human remains to be determined. Thus, the aim of this study was to examine cytotoxicity, antiproliferative, and antimutagenic actions of this extract. The hydroalcoholic extract from P. ramiflora leaves consisted of flavonoids identified and quantified as myricetin-3-O--D-galactopyranoside (13.55mg/g) and myricetin-3-O--L-rhamnopyranoside (9.61mg/g). The extract exhibited cytotoxicity at concentrations higher than 1.5 mu g/ml in human hepatocarcinoma (HepG2)and 2.5 mu g/ml in non-tumoral primary gastric (GAS) cells using the MTT assay, and at concentrations higher than 3 mu g/ml in HepG2 and 3.5 mu g/ml in GAS cells by the neutral red assay. The extract did not show antiproliferative effect as evidenced by the nuclear division index (NDI). However, in the presence of benzo{[}a]pyrene (BaP) (positive control), an enhanced cytostatic effect in the NDI and flow cytometry was noted. It is of interest that when the extract was co-incubated with BaP a significant decrease in DNA damage was observed indicating an antimutagenic action. This protective effect might be attributed to myricetin and gallic acid found in P. ramiflora extract. The low cytotoxicity action and protective effect observed in the present study encourage further studies regarding other biological effects of P. ramiflora, as well as its potential use as a chemopreventive agent. (AU)

• FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
• Grantee: Wagner Vilegas
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants