Tetracycline@silverions-functionalized mesoporous silica for high bactericidal activity at ultra-low concentration

Author(s)

Bouchmella, Karim ^{[1, 2, 3]} ; Campanaro, Felipe Davi ^{[1, 2]} ; Mondo, Gabriela Borba ^{[1, 2, 3]} ; Santos, Murilo Izidoro ^[2] ; Franco, Caio Haddad ^[4] ; Moraes, Carolina Borsoi ^[4] ; Biolley, Christine ^[5] ; Mehdi, Ahmad ^[5] ; Cardoso, Mateus Borba ^{[1, 2, 3]}

Affiliation

^[1] CNPEM, Lab Nacl Nanotecnol LNNano, BR-13083970 Campinas, SP - Brazil

^[2] CNPEM, LNLS, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas UNICAMP, IQ, Caixa Postal 6154, BR-13083970 Campinas, SP - Brazil

^[4] CNPEM, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, SP - Brazil

^[5] Univ Montpellier, CNRS, UMR5253, ICGM, ENSCM, F-34095 Montpellier - France

• Document type: Journal article
• Source: Nanomedicine; v. 13, n. 14, p. 1731-1751, JUL 2018.
• Web of Science Citations: 2

Abstract

Aim: Polyether pores were designed and tetracycline-loaded mesoporous silica materials, with their surface decorated by silver ions, were prepared, with the aim of reaching high antibacterial activity. Meanwhile, mammalian cell cytotoxicity and hemolytic effects were not observed using material concentrations tenfold the ones optimized for the bactericidal tests. Methods: Pore size was tuned by changing the polyether content and the surface was covalently decorated with silver thiolate groups. Results: We showed that the biological activity was enhanced by modulating silver ions and tetracycline releases by tuning silver thiolate group concentration on the silica surface and/or by modulating the pH of the environment. Conclusion: The combined use of tetracycline and silver ions with the mesoporous drug-delivery carrier was a very effective strategy against susceptible and tetracycline-resistant Escherichia coli bacteria. (AU)

• FAPESP's process: 14/22322-2 - Functionalization of silica nanoparticles: increasing biological interaction
• Grantee: Mateus Borba Cardoso
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/16905-0 - Colloidal stability and protein corona: fundamental aspects for maintaining silica nanoparticle properties in biologial media
• Grantee: Gabriela Borba Mondo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)