Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro production of conidia of Elsinoe ampelina, the causal fungus of grapevine anthracnose

Full text
Author(s):
dos Santos, Ricardo Feliciano [1] ; Sposito, Marcel Bellato [1] ; Ayres, Matthew [2] ; Sosnowski, Mark [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Fitopatol & Nematol, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil
[2] South Australian Res & Dev Inst, GPO Box 397, Adelaide, SA 5001 - Australia
Total Affiliations: 2
Document type: Journal article
Source: European Journal of Plant Pathology; v. 152, n. 3, p. 815-821, NOV 2018.
Web of Science Citations: 3
Abstract

Anthracnose, caused by Elsinoe ampelina, is an important disease of grapevines in humid and warm production regions of the world. Colonies of E. ampelina grow slowly and rarely produce conidia on artificial media. To facilitate studies involving E. ampelina, our objective was to develop a method to induce significant conidial production of this fungus. In the present study, we induced the in vitro production of conidia of 10 Australian isolates by shake-incubation of mycelial fragments in rainwater under continuous light and darkness. Furthermore, seven Brazilian isolates were shake-incubated in rainwater and distilled water under continuous darkness. In both experiments cultures were shaken at 200-rpm and kept at room temperature (22-25 degrees C) for 7days. Conidial production, germination, and severity of symptoms were quantified. Australian and Brazilian isolates produced different amounts of conidia. All Australian isolates sporulated in rainwater ranging from 4.20x10(3) to 5.91x10(6) conidia mL(-1), with more than 90% conidial germination on water agar medium. All but one Brazilian isolate produced conidia in rainwater and distilled water, ranging from 7.50x10(3) to 8.22x10(6) conidia mL(-1), with high germination percentages. Conidial suspensions from both Australian and Brazilian E. ampelina isolates caused typical anthracnose symptoms on grapevine leaves. This study describes an efficient method, using rainwater or distilled water associated with shaking, to induce the conidia production of E. ampelina. (AU)

FAPESP's process: 14/24472-1 - Etiology and epidemiology of anthracnose of grapevine
Grantee:Ricardo Feliciano dos Santos
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/01508-6 - Characterization of Elsinoe ampelina associated with anthracnose of grapevine in Brazil and Australia
Grantee:Ricardo Feliciano dos Santos
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/24003-9 - Grape diseases: epidemiology, damage assessment and control
Grantee:Lilian Amorim
Support Opportunities: Research Projects - Thematic Grants