Determination of Parabens in Breast Milk Samples by Dispersive Liquid-Liquid Microextraction (DLLME) and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

Author(s)

Grecco, Caroline F. ^[1] ; Souza, Israel D. ^[1] ; Acquaro Junior, Vinicius R. ^[1] ; Queiroz, Maria E. C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Av Bandeirantes 3900, BR-14040900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 30, n. 1, p. 48-59, JAN 2019.
• Web of Science Citations: 2

Abstract

A reliable method was developed and validated based on dispersive liquid-liquid microextraction (DLLME) and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) to determine simultaneously methylparaben (MePB), ethylparaben (EtPB), propylparaben (PrPB), and butylparaben (BuPB) in breast milk samples. A central composite design 24 with four independent variables and five levels of variance was used to optimize the DLLME conditions. The DLLME/UHPLC-MS/MS method was linear from 5 (lower limit of quantification, LLOQ) to 100 ng mL-1 (upper limit of quantification, ULOQ). The inter-assay and intra-assay precision values presented coefficients of variation (CVs) lower than 15%, and the relative standard error of the inter- and intra-assay accuracy values was lower than 10.4%. The DLLME/UHPLC-MS/MS method was successfully applied to determine parabens in human milk samples obtained from sixteen volunteer lactating mothers. (AU)

• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/04060-3 - Dispersive liquid-liquid microextraction of parabens in human milk samples and analysis by liquid chromatography-tandem mass spectrometry
• Grantee: Caroline Fernandes Grecco
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/07619-1 - Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids and drugs in biological samples
• Grantee: Maria Eugênia Queiroz Nassur
• Support Opportunities: Regular Research Grants