Rotary jet-spun porous microfibers as scaffolds for stem cells delivery to central nervous system injury

Author(s)

Zamproni, Laura N. ^{[1, 2]} ; Grinet, Marco A. V. M. ^[3] ; Mundim, Mayara T. V. V. ^{[1, 2]} ; Reis, Marcella B. C. ^{[1, 2]} ; Galindo, Layla T. ^{[1, 2]} ; Marciano, Fernanda R. ^[4] ; Lobo, Anderson O. ^{[5, 4]} ; Porcionatto, Marimelia ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Paulo, Neurobiol Lab, BR-04039032 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, Sao Paulo, SP - Brazil

^[3] Inst Tecnol Aeronaut, Sao Jose Dos Campos, SP - Brazil

^[4] Univ Brasil, Inst Ciencia & Tecnol, BR-08230030 Sao Paulo, SP - Brazil

^[5] Univ Fed Piaui, Programa Posgrad Ciencia & Engn Mat, Teresina, PI - Brazil

• Document type: Journal article
• Source: Nanomedicine-Nanotechnology Biology and Medicine; v. 15, n. 1, p. 98-107, JAN 2019.
• Web of Science Citations: 4

Abstract

Stem cell transplantation is a promising strategy to treat brain injuries. However, cell-based therapies are limited because poor local cell engraftment. Here, we present a polylactic acid (PLA) scaffold to support mesenchymal stem cells (MSCs) delivery in stroke. We isolated bone marrow MSCs from adult C57/B16 mice, cultured them on PLA polymeric rough microfibrous (PRM) scaffolds obtained by rotary jet spinning, and transplanted over the brains of adult C57/B16 mice, carrying thermocoagulation-induced cortical stroke. No inflammatory response to PRM was found. MSCs transplantation significantly reduced the area of the lesion and PRM delivery increased MSCs retention at the injury site. In addition, PRM upregulated alpha 6-integrin and CXCL12 production, which may be the cause for greater cell retention at the lesion site and may provide additional benefit to MSCs transplantation procedures. We conclude that PRM scaffolds offer a promising new system to deliver stem cells to injured areas of the brain. (C) 2018 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 12/00652-5 - Molecular mechanisms of neural stem cells migration, survival and differentiation
• Grantee: Marimélia Aparecida Porcionatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/05700-5 - STUDY OF THE NEUROGENIC EFFECTS PROMOTED BY MESENCHIMAL STEM CELLS EXPRESSING SDF-1 N-TERMINAL PEPTIDES TRANSPLANTED ON CENTRAL NERVOUS SYSTEM LESION
• Grantee: Marcella Braga da Costa Reis
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
• Grantee: Fernanda Roberta Marciano
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
• Grantee: Anderson de Oliveira Lobo
• Support Opportunities: Research Grants - Young Investigators Grants