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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spread of the emerging equine-like G3P[8] DS-1-like genetic backbone rotavirus strain in Brazil and identification of potential genetic variants

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Luchs, Adriana [1] ; da Costa, Antonio Charlys [2, 3] ; Cilli, Audrey [1] ; Vasconcelos Komninakis, Shirley Cavalcante [4, 5] ; Compagnoli Carmona, Rita de Cassia [1] ; Boen, Lais [1] ; Morillo, Simone Guadagnucci [1] ; Sabino, Ester Cerdeira [2, 3] ; Sampaio Tavares Timenetsky, Maria do Carmo [1]
Total Authors: 9
[1] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Infect & Parasit Dis, Coll Med, Lab Med Parasitol LIM 46, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Retrovirol Lab, Sao Paulo - Brazil
[5] Fac Med ABC, Postgrad Program Hlth Sci, Santo Andre - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF GENERAL VIROLOGY; v. 100, n. 1, p. 7-25, JAN 2019.
Web of Science Citations: 5

In 2013, the equine-like G3P{[}8] DS-1-like rotavirus (RVA) strain emerged worldwide. In 2016, this strain was reported in northern Brazil. The aims of the study were to conduct a retrospective genetic investigation to identify the possible entry of these atypical strains in Brazil and to describe their distribution across a representative area of the country. From 2013 to 2017, a total of 4226 faecal samples were screened for RVA by ELISA, PAGE, RT-PCR and sequencing. G3P{[}8] represented 20.9 % (167/800) of all RVA-positive samples, further subdivided as equine-like G3P{[}8], DS-1-like (11.0 %; 88/800) and Wa-like G3P{[}8] (9.9%; 79/800). Six equine-like G3P{[}8] DS-1-like samples were selected for whole-genome investigation, confirming the backbone I2 R2 C2 M2 A2 N2 T2 E2 H2. During 2013-2014, Wa-like G3P{[}8] was predominant and no equine-like G3P{[}8] DS-1-like was detected. Equine-like G3P{[}8] DS-1-like was first identified in Parana in March/2015, suggesting that the strain entered Brazil through the Southern region. Equine-like G3P{[}8] rapidly spread across the area under surveillance and displayed a marked potential to replace Wa-like G3P{[}8] strains. Brazilian equine-like G3P{[}8] DS-1-like strains clustered with contemporary equine-like G3P{[}8] DS-1-like detected worldwide, but exhibited a distinct NSP2 genotype (N2) compared to the previously reported Amazon equine-like G3P{[}8] DS-1-like strain (N1). Two distinct NSP4 E2 genotype lineages were also identified. Taken together, these data suggest that different variants of equine-like G3P{[}8] DS-1-like strains might have been introduced into the country at distinct time points, and co-circulated in the period 2015-2017. The global emergence of equine-like G3P{[}8] DS-1-like strains, predominantly in countries using the Rotarix vaccine, raises the question of whether vaccines may be inducing selective pressures on zoonotic strains. (AU)

FAPESP's process: 15/12944-9 - Investigating the evoltution of animal rotavirus strains infecting humans
Grantee:Adriana Luchs
Support type: Regular Research Grants
FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
Grantee:Antonio Charlys da Costa
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/01735-2 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
Grantee:Ester Cerdeira Sabino
Support type: Regular Research Grants