Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs

Author(s)

Gallinari, Marjorie de Oliveira ^[1] ; Angelo Cintra, Luciano Tavares ^[1] ; Benetti, Francine ^[1] ; Rahal, Vanessa ^[1] ; Ervolino, Edilson ^[2] ; Fraga Briso, Andre Luiz ^[1]

Affiliation

^[1] UNESP Univ Estadual Paulista, Aracatuba Dent Sch, Dept Restorat Dent, Aracatuba, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 14, n. 1 JAN 8 2019.
• Web of Science Citations: 2

Abstract

This study aimed to evaluate neuropeptide expression after bleaching treatment using histopathological and immunohistochemical analyses and the effects of hydrocortisone and acetaminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and only then, the treatmentt. Sixty-three rats were divided into three groups (n = 21) according to the pain-relieving therapy used: I-control; II-topical application of Otosporin for 10 min after the bleaching treatment; Ill-oral administration of paracetamol 30 min before whitening and then every 12h. In all the study groups, placebo gel was applied to the left upper jaw (control) and a 35% H2O2-based whitening gel was applied to the right upper jaw for 45 min. Seven animals from each group were euthanized at different time points: 0h after treatment, 24h, and 48h. After euthanasia, the first molar on each side was analyzed by histology and immunohistochemistry to assess the degree of inflammation and verify the presence of the neuropeptides, substance P (SP) and calcitonin gene-related peptide (CGRP). The data were analyzed using the statistical nonparametric Kruskal-Wallis test followed by Dunn's test for individual comparisons. Extensive areas of necrosis were observed in the groups that received bleaching treatment only, whereas reduced damage were obtained in the group treated with Otosporin. The immunohistochemical analysis showed positive immuno-labeling in all groups, including the control, but this was stronger in the groups that received bleaching treatment. The best results were obtained in the group that received treatment with Otosporin. The use of Otosporin after dental bleaching minimized the side effects of this treatment. (AU)

• FAPESP's process: 15/01366-4 - Influence of the bleaching agents compositions in inflammation and neuropeptide expression after bleaching
• Grantee: André Luiz Fraga Briso
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/21682-8 - Influence of the use of topical and systemic substances in inflammation after bleaching treatment
• Grantee: Livia Maria Alves Valentim da Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation