| Full text | |
| Author(s): |
Brunetti, Andres E.
[1]
;
Lyra, Mariana L.
[2, 3]
;
Melo, Weilan G. P.
[1]
;
Andrade, Laura E.
[1]
;
Palacios-Rodriguez, Pablo
[4]
;
Prado, Barbara M.
[1]
;
Haddad, Celio F. B.
[2, 3]
;
Pupo, Monica T.
[1]
;
Lopes, Norberto P.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Estadual Paulista, Inst Biociencias, Dept Zool, BR-13506900 Rio Claro, SP - Brazil
[3] Univ Estadual Paulista, Inst Biociencias, Ctr Aquicultura, BR-13506900 Rio Claro, SP - Brazil
[4] Univ Los Andes, Dept Ciencias Biol, Bogota 4976 - Colombia
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; v. 116, n. 6, p. 2124-2129, FEB 5 2019. |
| Web of Science Citations: | 2 |
| Abstract | |
Amphibians are known to possess a wide variety of compounds stored in their skin glands. While significant progress has been made in understanding the chemical diversity and biological relevance of alkaloids, amines, steroids, and peptides, most aspects of the odorous secretions are completely unknown. In this study, we examined sexual variations in the volatile profile from the skin of the tree frog Boana prasina and combined culture and culture-independent methods to investigate if microorganisms might be a source of these compounds. We found that sesquiterpenes, thioethers, and methoxypyrazines are major contributors to the observed sex differences. We also observed that each sex has a distinct profile of methoxypyrazines, and that the chemical origin of these compounds can be traced to a Pseudomonas sp. strain isolated from the frog's skin. This symbiotic bacterium was present in almost all individuals examined from different sites and was maintained in captive conditions, supporting its significance as the source of methoxypyrazines in these frogs. Our results highlight the potential relevance of bacteria as a source of chemical signals in amphibians and contribute to increasing our understanding of the role that symbiotic associations have in animals. (AU) | |
| FAPESP's process: | 14/20915-6 - Chemical ecology in anurans: characterization of volatile compounds and peptide pheromones from the skin secretions of frogs |
| Grantee: | Andrés Eduardo Brunetti |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 15/01001-6 - Discovering bacterial symbionts diversity associated with ants in different Brazilian biomes |
| Grantee: | Weilan Gomes da Paixão Melo |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 13/50741-7 - Diversity and conservation of Brazilian amphibians |
| Grantee: | Célio Fernando Baptista Haddad |
| Support type: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 17/26162-8 - Diversity and conservation of Brazilian amphibians |
| Grantee: | Mariana Lúcio Lyra |
| Support type: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 17/23725-1 - Genomic Analysis of Skin Symbiotic Bacteria in Frogs as Potential Source of Volatile Compounds |
| Grantee: | Andrés Eduardo Brunetti |
| Support type: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 14/50265-3 - Distribution and metabolism of natural and synthetic xenobiotics: from the comprehension of reactional process to tissue imaging generation |
| Grantee: | Norberto Peporine Lopes |
| Support type: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 13/50954-0 - Novel therapeutic agents from the bacterial symbionts of Brazilian invertebrates |
| Grantee: | Mônica Tallarico Pupo |
| Support type: | BIOTA-FAPESP Program - Thematic Grants |