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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Self-Assembling Peptide P-11-4 Prevents Collagen Proteolysis in Dentin

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de Sousa, J. P. [1] ; Carvalho, R. G. [2] ; Barbosa-Martins, L. F. [1] ; Torquato, R. J. S. [2] ; Mugnol, K. C. U. [3] ; Nascimento, F. D. [3] ; Tersariol, I. L. S. [2] ; Puppin-Rontani, R. M. [1]
Total Authors: 8
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Campinas, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo, SP - Brazil
[3] Univ Mogi das Cruzes, Interdisciplinary Ctr Biochem Invest, BR-08773520 Mogi Das Cruzes, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF DENTAL RESEARCH; v. 98, n. 3, p. 347-354, MAR 2019.
Web of Science Citations: 1

The major goal in restorative dentistry is to develop a true regenerative approach that fully recovers hydroxyapatite crystals within the caries lesion. Recently, a rationally designed self-assembling peptide P-11-4 (Ace-QQRFEWEFEQQ-NH2) has been developed to enhance remineralization on initial caries lesions, yet its applicability on dentin tissues remains unclear. Thus, the present study investigated the interaction of P-11-4 with the organic dentin components as well as the effect of P-11-4 on the proteolytic activity, mechanical properties of the bonding interface, and nanoleakage evaluation to artificial caries-affected dentin. Surface plasmon resonance and atomic force microscopy indicated that P-11-4 binds to collagen type I fibers, increasing their width from 214 +/- 4 nm to 308 +/- 5 nm (P < 0.0001). P-11-4 also increased the resistance of collagen type I fibers against the proteolytic activity of collagenases. The immediate treatment of artificial caries-affected dentin with P-11-4 enhanced the microtensile bonding strength of the bonding interface (P < 0.0001), reaching values close to sound dentin and decreasing the proteolytic activity at the hybrid layer; however, such effects decreased after 6 mo of water storage (P < 0.05). In conclusion, P-11-4 interacts with collagen type I, increasing the resistance of collagen fibers to proteolysis, and improves stability of the hybrid layer formed by artificial caries-affected dentin. (AU)

FAPESP's process: 14/22899-8 - Effect of remineralizing agents on proteolytic activity of caries-affected dentin and on and resin/dentin bond
Grantee:Jossaria Pereira de Sousa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/12660-0 - Effect of remineralizing agents on proteolytic activity and resin/caries-affected dentin bond longevity
Grantee:Regina Maria Puppin Rontani
Support type: Regular Research Grants
FAPESP's process: 15/03964-6 - Glycosaminoglycans and proteoglycans: interplay between structure and function
Grantee:Helena Bonciani Nader
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/05822-9 - Proteinase-Activated Receptors (PARs) in the dentin-pulp complex: identification, modulation and signal tranduction in caries disease
Grantee:Fábio Dupart Nascimento
Support type: Research Grants - Young Investigators Grants