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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232

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Espinal, P. [1, 2] ; Nucleo, E. [3] ; Caltagirone, M. [3] ; Marchetti, V. Mattioni [3] ; Fernandes, M. R. [4, 1] ; Biscaro, V [5] ; Rigoli, R. [5] ; Carattoli, A. [1] ; Migliavacca, R. [3] ; Villa, L. [1]
Total Authors: 10
[1] Ist Super Sanita, Dept Infect Dis, Viale Regina Margherita 299, I-00161 Rome - Italy
[2] Inst Invest Biomed St Pau IIB St Pau, Servei Microbiol Hosp Santa Creu & St Pau, Barcelona - Spain
[3] Univ Pavia, Unit Microbiol & Clin Microbiol, Clin Surg Diagnost & Paediat Sci Dept, Pavia - Italy
[4] Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo - Brazil
[5] Treviso Hosp, Microbiol Dept, Treviso - Italy
Total Affiliations: 5
Document type: Journal article
Source: Clinical Microbiology and Infection; v. 25, n. 3 MAR 2019.
Web of Science Citations: 3

Objectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenemresistant Klebsiella pneumoniae. Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, core genome multilocus sequence typing, resistance, plasmid, and virulence content and compared with ten genomes of ST16 strains isolated in other countries. Plasmids carrying blaNDM-1 or blaOXA-232 carbapenemase genes were assembled and sequences were analysed. Results: The internationally spread ST16 K. pneumoniae clone showed variability in terms of distribution of NDM-1 and OXA-232 type carbapenemases. In some ST16 strains, up to six plasmids can be simultaneously present in the same cell, including ColE-like plasmids carrying blaOXA-232 and IncF plasmids carrying blaNDM-1. The differences observed in plasmid, resistance, and virulence content and core genome suggested that there is not a unique, highly conserved ST16 clone, but instead different variants of this lineage circulate worldwide. Conclusions: The ST16 K. pneumoniae clone has spread worldwide and may become a high-risk clone. (c) 2018 Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. (AU)

FAPESP's process: 17/16754-5 - Characterization of plasmids associated with the spread of the colistin resistance MCR-1 gene in clinically significant Enterobacteriaceae from South American and European Countries
Grantee:Miriam Rodriguez Fernandes
Support type: Scholarships abroad - Research Internship - Doctorate