| Full text | |
| Author(s): Show less - |
Di Paola, Nicholas
[1]
;
Mesquita, Flavio S.
[1]
;
Leal de Oliveira, Danielle Bruna
[1]
;
Villabona-Arenas, Christian Julian
[2]
;
Pour, Shahab Zaki
[1]
;
de Sousa-Capra, Carla
[3]
;
Lopes, Gabriela Pessanha
[4]
;
Ferraz Santana, Rubia Anita
[4]
;
Rebello Pinho, Joao Renato
[4, 5]
;
Balarini, Karime
[6]
;
Theoto Pereira da Fonseca, Celso Ricardo
[6]
;
de Andrade Zanotto, Paolo Marinho
[1]
Total Authors: 12
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Ave Prof Lineu Prestes 1374, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Montpellier, INSERM, Inst Rech Dev, Rech Translat Virus Immunodeficience & Malad Infe, Montpellier - France
[3] Dept Hlth Guaruja, Off Epidemiol Surveillance, Sao Paulo - Brazil
[4] Hosp Israelita Albert Einstein, Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Med, Lab Gastroenterol Clin & Expt, Lab Med Lab, Sao Paulo - Brazil
[6] Itapema Lab Anal Clin, Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Clinical Infectious Diseases; v. 68, n. 5, p. 810-817, MAR 1 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Background. Seasonal outbreaks of dengue often result in hundreds of dengue-suspected cases where a clinical diagnosis cannot be confirmed. Usually, during large outbreaks of dengue and other pathogens that can cause acute febrile illnesses, the search for secondary pathogens with similar disease outcomes is rare. Methods. Using total RNA sequencing and targeted diagnostic assays, we discovered an outbreak of parvovirus B19 in dengue-suspected patients that occurred from November 2013 to February 2014. Results. Of the 182 cases investigated, 63% were viremic for the B19 virus. Moreover, we found that >43% of infected patients had no serological evidence of prior infection. Parvovirus B19 is a typical childhood infection, yet we observed that 82% of the infected patients were adults. Additionally, we perceived that infected adults had significantly higher presentations of myalgia than in children. We also obtained viral protein (VP) 1/VP2 gene nucleotide sequences from 43 patients. Conclusions. Our results support the utility of next-generation sequencing for symptomatic patients with unknown etiologies during seasonal outbreaks of dengue and other arborviruses. Our findings could improve the vigilance of hospitals and laboratories by raising awareness of co-circulating pathogens such as parvovirus B19 that may be hidden in plain sight. (AU) | |
| FAPESP's process: | 14/17766-9 - A systemic approach to study permissivity on the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) |
| Grantee: | Paolo Marinho de Andrade Zanotto |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/25643-7 - Molecular characterization and epidemiologic profile of human adenovirus infection in pediatric patients with acute respiratory infection in São Paulo |
| Grantee: | Flávio da Silva Mesquita |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 13/22136-1 - Discovering novel viruses in communities encroaching tropical hotspots via a metagenomics approach |
| Grantee: | Nicholas Di Paola |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |