Real-time, rapid detection and sequencing of arboviruses in Brazil: Zika, Dengue, Chikungunya and Yellow Fever

• Grant number: 18/17176-8
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: January 01, 2019
• End date: October 31, 2021
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Ester Cerdeira Sabino
• Grantee: Ingra Morales Claro
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 19/25047-6 - Establishing a rapid and mobile approach to detect new epidemics, BE.EP.DD

Abstract

Arbovirus infections in Brazil including Zika, Chikungunya, Dengue and Yellow Fever result inconsiderable morbidity and mortality and are pressing public health concerns. However ourunderstanding of these outbreaks is hampered by limited availability of epidemiological surveillance data. Typically these data are generated by molecular diagnostic assays that are expensive to setup and lack specificity. Consequently we have limited understanding of the interplay and interactions between arboviruses in both human and mosquito populations in Brazil, as demonstrated by Zika Virus in Brazil where detection was delayed by at least a year from the true start of the outbreak. Recently we pioneered a new approach to surveillance of arboviruses using portable, real-time nanopore sequencing - so-called 'genomic epidemiology'. This permits sensitive detection and assignment of viruses into genotypes. However, at present, the costs and complexity of this powerful technique (around $100) currently precludes its use as a routine surveillance tool in research and public health laboratory environments. In this proposal we aim to solve three pressing problems with portable sequencing for arbovirusdetection. This continues a highly productive collaboration between the University of São Paulo, the University of Birmingham and the University of Nottingham that has already resulted in very high impact publications in Nature and Nature Protocols (Faria et al. 2017; Quick et al. 2017). This project will have an immediate impact on the surveillance of arboviruses in Brazil and our understanding of this pressing public health problem. (AU)

Scientific publications

(8)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

THAÍS DE MOURA COLETTI; CAMILA MALTA ROMANO; PAULO ROBERTO URBINATTI; RENILDO SOUZA TEIXEIRA; LEILA WEISS DE ALMEIDA PEDROSA; MARCELLO SCHIAVO NARDI; DELSIO NATAL; ANTÔNIO CHARLYS DA COSTA; JAQUELINE GOES DE JESUS; INGRA MORALES CLARO; et al. Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 55, 2022-09-26. (17/00021-9, 18/17176-8, 19/03859-9, 18/14389-0, 16/18204-0, 19/12000-1, 10/51230-8)

CANDIDO, DARLAN S.; CLARO, INGRA M.; DE JESUS, JAQUELINE G.; SOUZA, WILLIAM M.; MOREIRA, FILIPE R. R.; DELLICOUR, SIMON; MELLAN, THOMAS A.; DU PLESSIS, LOUIS; PEREIRA, RAFAEL H. M.; SALES, FLAVIA C. S.; et al. Evolution and epidemic spread of SARS-CoV-2 in Brazil. Science, v. 369, n. 6508, SI, p. 1255+, SEP 4 2020. (20/04558-0, 16/00194-8, 19/21301-5, 18/14389-0, 18/14933-2, 17/13981-0, 20/04836-0, 18/25468-9, 16/18445-7, 18/09383-3, 18/17176-8, 19/12000-1, 19/07544-2, 19/24251-9)

DE SOUZA, WILLIAM M.; FUMAGALLI, MARCILIO J.; DE LIMA, SHIRLENE T. S.; PARISE, PIERINA L.; CARVALHO, DEYSE C. M.; HERNANDEZ, CRISTIAN; DE JESUS, RONALDO; DELAFIORI, JEANY; CANDIDO, DARLAN S.; CARREGARI, VICTOR C.; et al. Pathophysiology of chikungunya virus infection associated with fatal outcomes. CELL HOST & MICROBE, v. 32, n. 4, p. 26-pg., 2024-04-10. (19/24251-9, 17/26908-0, 22/04420-3, 18/14389-0, 19/27333-6, 19/05155-9, 18/17176-8, 17/25588-1, 19/00098-7, 13/08293-7, 19/05718-3)

FORATO, JULIA; MEIRA, CASSIO A.; CLARO, INGRA M.; AMORIM, MARIENE R.; DE SOUZA, GABRIELA F.; MURARO, STEFANIE P.; TOLEDO-TEIXEIRA, DANIEL A.; DIAS, MIGUEL F.; MENESES, CATIA A. R.; ANGERAMI, RODRIGO N.; et al. Molecular Epidemiology of Mayaro Virus among Febrile Patients, Roraima State, Brazil, 2018-2021. Emerging Infectious Diseases, v. 30, n. 5, p. 4-pg., 2024-05-01. (18/14389-0, 16/00194-8, 18/17176-8, 22/10442-0, 20/04558-0)

COLETTI, THAIS DE MOURA; ROMANO, CAMILA MALTA; URBINATTI, PAULO ROBERTO; TEIXEIRA, RENILDO SOUZA; DE ALMEIDA PEDROSA, LEILA WEISS; NARDI, MARCELLO SCHIAVO; NATAL, DELSIO; DA COSTA, ANTONIO CHARLYS; DE JESUS, JAQUELINE GOES; CLARO, INGRA MORALES; et al. Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in Sao Paulo, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 55, p. 5-pg., 2022-01-01. (18/14389-0, 16/18204-0, 18/17176-8, 10/51230-8, 19/03859-9, 19/12000-1, 17/00021-9)

IANI, FELIPE C. M.; GIOVANETTI, MARTA; FONSECA, VAGNER; SOUZA, WILLIAM M.; ADELINO, TALITA E. R.; XAVIER, JOILSON; JESUS, JAQUELINE G.; PEREIRA, MAIRA A.; SILVA, MARCOS V. F.; COSTA, ALANA V. B.; et al. Epidemiology and evolution of Zika virus in Minas Gerais, Southeast Brazil. INFECTION GENETICS AND EVOLUTION, v. 91, JUL 2021. (18/14389-0, 17/13981-0, 18/17176-8, 19/12000-1, 19/24251-9)

FARIA, NUNO R.; MELLAN, THOMAS A.; WHITTAKER, CHARLES; CLARO, INGRA M.; CANDIDO, DARLAN DA S.; MISHRA, SWAPNIL; CRISPIM, MYUKI A. E.; SALES, FLAVIA C.; HAWRYLUK, IWONA; MCCRONE, JOHN T.; et al. Genomics and epidemiology of the P.1 SARS-CoV-2 lineage in Manaus, Brazil. Science, v. 372, n. 6544, p. 815+, MAY 21 2021. (20/04558-0, 19/21568-1, 19/21301-5, 18/14389-0, 17/13981-0, 20/04272-9, 18/25468-9, 16/18445-7, 19/21858-0, 18/17176-8, 19/12000-1, 19/07544-2, 18/12579-7, 19/24251-9)

FERREIRA, GIULIA MAGALHAES; CLARO, INGRA MORALES; GROSCHE, VICTORIA RIQUENA; CANDIDO, DARLAN; JOSE, DIEGO PANDELO; ROCHA, ESMENIA COELHO; COLETTI, THAIS DE MOURA; MANULI, ERIKA REGINA; GABURO JR, NELSON; FARIA, NUNO RODRIGUES; et al. Molecular characterization and sequecing analysis of SARS-CoV-2 genome in Minas Gerais, Brazil. BIOLOGICALS, v. 80, p. 10-pg., 2022-10-01. (18/17176-8, 18/14389-0, 19/12000-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CLARO, Ingra Morales. Real-time, rapid detection and sequencing of arboviruses in Brazil. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.