Fish Oil Protects Wild Type and Uncoupling Protein 1-Deficient Mice from Obesity and Glucose Intolerance by Increasing Energy Expenditure

Author(s):Show less -

Oliveira, Tiago E. ^[1] ; Castro, Erique ^[1] ; Belchior, Thiago ^[1] ; Andrade, Maynara L. ^[1] ; Chaves-Filho, Adriano B. ^[1] ; Peixoto, Albert S. ^[1] ; Moreno, Mayara F. ^[1] ; Ortiz-Silva, Milene ^[1] ; Moreira, Rafael J. ^[1] ; Inague, Alex ^[2] ; Yoshinaga, Marcos Y. ^[2] ; Miyamoto, Sayuri ^[2] ; Moustaid-Moussa, Naima ^{[3, 4]} ; Festuccia, William T. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil

^[3] Texas Tech Univ, Dept Nutr Sci, Lubbock, TX 79409 - USA

^[4] Texas Tech Univ, Obes Res Cluster, Lubbock, TX 79409 - USA

• Document type: Journal article
• Source: MOLECULAR NUTRITION & FOOD RESEARCH; v. 63, n. 7 APR 2019.
• Web of Science Citations: 4

Abstract

Scope The mechanisms and involvement of uncoupling protein 1 (UCP1) in the protection from obesity and insulin resistance induced by intake of a high-fat diet rich in omega-3 (n-3) fatty acids are investigated. Methods and results C57BL/6J mice are fed either a low-fat (control group) or one of two isocaloric high-fat diets containing either lard (HFD) or fish oil (HFN3) as fat source and evaluated for body weight, adiposity, energy expenditure, glucose homeostasis, and inguinal white and interscapular brown adipose tissue (iWAT and iBAT, respectively) gene expression, lipidome, and mitochondrial bioenergetics. HFN3 intake protected from obesity, glucose and insulin intolerances, and hyperinsulinemia. This is associated with increased energy expenditure, iWAT UCP1 expression, and incorporation of n-3 eicosapentaenoic and docosahexaenoic fatty acids in iWAT and iBAT triacylglycerol. Importantly, HFN3 is equally effective in reducing body weight gain, adiposity, and glucose intolerance and increasing energy expenditure in wild-type and UCP1-deficient mice without recruiting other thermogenic processes in iWAT and iBAT, such as mitochondrial uncoupling and SERCA-mediated calcium and creatine-driven substrate cyclings. Conclusion Intake of a high-fat diet rich in omega-3 fatty acids protects both wild-type and UCP1-deficient mice from obesity and insulin resistance by increasing energy expenditure through unknown mechanisms. (AU)

• FAPESP's process: 15/19530-5 - Involvement of the nutrient sensor mTOR in the development of obesity associated chronic metabolic diseases
• Grantee: William Tadeu Lara Festuccia
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/17582-3 - Characterization of the involvement of nutrient sensor mTORC1 in the hepatic steatosis, steatohepatitis, and hepatocarcinoma induced by PTEN deletion into hepatocytes
• Grantee: Mayara Franzoi Moreno
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/07062-0 - Characterization of the mechanisms underlying omega-3 fatty acids effects on energy balance
• Grantee: Tiago Eugênio Oliveira da Silva
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/12260-8 - Autophagy involvement in the metabolic and inflammatory alterations in adipose tissue associated with Obesity induced by high fat diet
• Grantee: Milene Ortiz Silva
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/22983-1 - Discovery of new transcription factors involved in the development of steatosis, steatohepatitis, and liver cancer
• Grantee: Thiago Belchior de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/23169-9 - Involvement of mTORC1 and 2 and PPARgamma in the regulation of leukocyte profile, recruitment and activation of brown adipose tissue and beige adipocytes induced by cold exposure
• Grantee: Érique de Castro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/13508-8 - Involvement of adipocytes mTOR complexes 1 and 2 in the morphological, metabolic and secretory changes induced by pharmacological PPARgamma activation
• Grantee: Maynara Lucca Andrade
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/11156-5 - Effects of obesity and severe lipodystrophy on oxidation and peroxidation of lipids: involvement of these molecules on inflammation and insulin resistance
• Grantee: Adriano de Britto Chaves Filho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/23040-9 - Characterization of mTORC2 role in the hepatic steatosis, steatohepatitis and hepatocellular carcinoma progression induced by PTEN deletion in hepatocytes
• Grantee: Álbert Souza Peixoto
• Support Opportunities: Scholarships in Brazil - Doctorate