| Full text | |
| Author(s): |
Teixeira, Luciane de L.
[1, 2]
;
Dorr, Fabiane
[3, 2]
;
Dias, Carlos T. S.
[4]
;
Pinto, Ernani
[3, 2]
;
Lajolo, Franco M.
[1, 2]
;
Villas-Boas, Silas G.
[5]
;
Hassimotto, Neuza M. A.
[1, 2]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Nutr, Sao Paulo - Brazil
[2] CEPID FAPESP, Res Innovat & Disseminat Ctr, Sao Paulo Res Fdn, Food Res Ctr FoRC, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Agr Luiz de Queiroz, Piracicaba - Brazil
[5] Univ Auckland, Sch Biol Sci, Ctr Microbial Innovat, Auckland - New Zealand
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Food Research International; v. 120, p. 544-552, JUN 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Polyphenol intake has been associated with health promotion because of its interaction with several metabolic pathways. This study investigates changes in the urine metabolome following acute intake of polyphenol-rich juice, purple grumixama juice. Grumixama (Eugenia brasiliensis Lam.) is a cherry native to Brazil that is known to be a rich source of anthocyanins and ellagitannins. In this research 15 healthy subjects consumed a single dose of grumixama juice. Urine samples were collected before grumixama juice intake, 0-1, 1-2, 2-4 h, with fasting at 24 h after intake. Plasma samples were also collected before intake, 30' and at 1 h, 2 h and 4 h, with fasting at 24 h after juice intake. The urine primary metabolites were analysed by a metabolomic approach using gas chromatography mass spectrometry with methyl chloroformate derivatisation for amino acids and organic acids. Also, an oxygen radical absorbance capacity method was carried out to evaluate the plasma samples antioxidant capacity changes. Subjects showed increase in plasma antioxidant capacity after juice intake (p-values < .05). A total of 114 metabolites were assessed in urine (1-2 h and 2-4 h), including 17 amino acids, 47 organic acids and several other metabolites. Among the 114 metabolites, 25 were significantly changed during the first 4 h following juice intake, as shown by the Orthogonal Partial Least Squares Discriminant Analysis (0.5 > p (corr) > 0.3) and univariate analysis (p-values < .05). Some metabolites were related to mitochondrial metabolism, such as glyoxylic acid and oxalic acid. Metabolites related to amino acid metabolism were also changed, such as beta-alanine, L-phenylalanine and L-tyrosine. In conclusion, results suggest that acute intake of grumixama juice could affect amino acid metabolism and mitochondrial metabolism, but the related health implications should be explored in further studies using additional approaches. (AU) | |
| FAPESP's process: | 13/07914-8 - FoRC - Food Research Center |
| Grantee: | Bernadette Dora Gombossy de Melo Franco |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |