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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the tubing material and physical dimensions on the performance of extraction columns for on-line sample preparation-LC-MS/MS

Full text
Author(s):
de Toffoli, Ana Lucia [1] ; Soares Maciel, Edvaldo Vasconcelos [1] ; Lancas, Fernando Mauro [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Chem Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Chromatography A; v. 1597, p. 18-27, JUL 19 2019.
Web of Science Citations: 0
Abstract

Nowadays, high analytical throughputs are required considering an increasing demand for faster, simple and improved methods to analyze contaminants in a considerable number of samples. Generally, these compounds are present in complex matrices in contact with a high number of interferents becoming their determination difficult at low concentration. In this context, on-line extraction techniques arose to improve the extraction as well as separation power, while minimizing errors related to human sample manipulation. This paper describes a study regarding the development and optimization of columns used as an extraction device in multidimensional liquid chromatography. The main goals were the evaluation of the material used as column body as well as the investigation of the tube dimensions (internal diameter and length) in the extraction performance. Firstly, several tube materials were tested (steel, fused silica, PEEK, among others) being steel whose reported the best performance and was consequently chose for further studies. The investigation about the effects of the columns physical dimensions revealed a linear relationship between performance and the amount of sorbent utilized as extractive phase. However, when different columns with same amount of sorbent were tested results suggests that both i.d. and lengths play an important role in extraction efficiency. The longest columns with lower internal diameter showed the best results favoring the radial as well as axial analytes diffusion into the extraction column. After evaluation of these column variables, applications were carried out employing several different analytes belonging to various chemical classes and practical utilization, in order to reinforce the versatility as well as the robustness of this proposed study. (C) 2019 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
Grantee:Fernando Mauro Lanças
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/15462-5 - Employment of graphene as sorbent in miniaturized sample preparation techniques.
Grantee:Bruno Henrique Fumes
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/07347-9 - Development of novel materials and devices for miniaturized sample preparation techniques in the area of food safety
Grantee:Fernando Mauro Lanças
Support Opportunities: Regular Research Grants