Pradimicin-IRD from Amycolatopsis sp. IRD-009 and its antimicrobial and cytotoxic activities

Author(s)

Bauermeister, Anelize ^[1] ; Calil, Felipe A. ^[1] ; Pinto, Francisco das C. L. ^[2] ; Medeiros, Talita C. T. ^[1] ; Almeida, Larissa C. ^[3] ; Silva, Leonardo J. ^[4] ; de Melo, Itamar S. ^[5] ; Zucchi, Tiago D. ^[6] ; Costa-Lotufo, Leticia V. ^[3] ; Moraes, Luiz A. B. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto - Brazil

^[2] Univ Fed Ceara, Dept Quim Organ & Inorgan, Fortaleza, Ceara - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Farmacol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Piracicaba - Brazil

^[5] Natl Brazilian Res Corp EMBRAPA ENVIRONM, Lab Environm Microbiol, Jaguariuna - Brazil

^[6] AGRIVALLE, Dept Pesquisa & Desenvolvimento, Salto - Brazil

• Document type: Journal article
• Source: NATURAL PRODUCT RESEARCH; v. 33, n. 12, p. 1713-1720, JUN 18 2019.
• Web of Science Citations: 2

Abstract

A new polycyclic antibiotic, pradimicin-IRD, was isolated from actinobacteria Amycolatopsis sp. IRD-009 recovered from soil of Brazilian rainforest undergoing restoration area. This molecule is the major compound produced in solid culture media. The new compound was detected by a focused method of precursor ion (high-performance liquid chromatography coupled to tandem mass spectrometer) developed previously to identify unusual aminoglycosyl sugar moieties. The compound was isolated and its structure was, therefore, elucidated by high-resolution mass spectrometry, and 1D and 2D nuclear magnetic resonance experiments. Pradimicin-IRD displayed potential antimicrobial activity against Streptococcus agalactiae (MIC 3.1 mu g/mL), Pseudomonas aeruginosa (MIC 3.1 mu g/mL) and Staphylococcus aureus (MIC 3.1 mu g/mL), and also cytotoxicity against tumour and non-tumour cell lines with IC50 values ranging from 0.8 mu M in HCT-116 colon carcinoma cells to 2.7 mu M in MM 200 melanoma cells. Particularly, these biological properties are described for the first time for this chemical class. {[}GRAPHICS] . (AU)

• FAPESP's process: 13/50729-7 - DESI-HPTLC-Bioautography: New strategies to rapid Identification of anti-mastotos bioactive metabolites from actinomycetes.
• Grantee: Luiz Alberto Beraldo de Moraes
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 17/17648-4 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds
• Grantee: Anelize Bauermeister
• Support Opportunities: Scholarships in Brazil - Post-Doctoral