| Full text | |
| Author(s): |
Miyazaki, Celina Massumi
[1]
;
Camilo, Douglas Eleuterio
[1]
;
Shimizu, Flavio Makoto
[2, 3]
;
Ferreira, Marystela
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Fed Sao Carlos, CCTS, Rd Joao Leme Santos, Km 110, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Applied Surface Science; v. 490, p. 502-509, OCT 1 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Immunoassays are important for the diagnosis of many diseases. Efficient methods for the immobilization of the biorecognition elements is a relevant subject because the antibody loading is directly related to the detection range and sensitivity. Here different self-assembled films were explored to take advantage of polar oxygenated groups from graphene oxide (GO) structure. Surface plasmon resonance (SPR) sensors were modified with different GO self-assembled films and compared to the well-established thiol-functionalization with 11-MUA (11-mercaptoundecanoic acid). Self-assembled cysteamine/GO functionalized sensor (Cys-GO(SA)) provided the highest protein loading (7.66 x 10(-12) mol cm(-2) against 1.29 x 10(-11) mol cm(-2) for the traditional thiolfunctionalization). The Langmuir-Blodgett (LB) films of GO were composed of aggregates and empty spaces, which hindered the antibody anchoring (around 6.38 x 10(-12) to 3.45 x 10(-12) mol cm(-2)). The SPR response of the Cys-GO(SA)-modified sensor to the 4.0 ng mL(-1) PSA was 125% higher than the thiol-functionalized sensor at the same concentration. This result indicates a simple and promising surface modification strategy for many important applications, not limited to SPR sensors, but for all immunoreaction-based assays as enzyme-linked immunosorbent assay (ELISA), electrochemical (e.g. amperometric and impedimetric) and quartz crystal microbalance (QCM) based sensors. (AU) | |
| FAPESP's process: | 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing |
| Grantee: | Celina Massumi Miyazaki |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/19387-0 - LbL (LAYER-BY-LAYER) and Langmuir-Blodgett (LB) nanostructured films with emodin for systems carriers. |
| Grantee: | Marystela Ferreira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/50869-6 - INCT 2014: on Organic Electronics |
| Grantee: | Roberto Mendonça Faria |
| Support Opportunities: | Research Projects - Thematic Grants |