Comparing antioxidant activities of flavonols from Annona coriacea by four approaches

Author(s)

Novaes, P. ^[1] ; Torres, P. B. ^[1] ; Cornu, T. A. ^[2] ; de Carvalho Lopes, J. ^[1] ; Ferreira, M. J. P. ^[1] ; dos Santos, D. Y. A. C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Bot, Inst Biosci, Rua Matao, 277 Cidade Univ, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Rennes 1, Ment Biol Agrosante Parcours Sci & Prod Vegetales, 9 Rue Jean Mace, F-35000 Rennes - France

• Document type: Journal article
• Source: SOUTH AFRICAN JOURNAL OF BOTANY; v. 123, p. 253-258, JUL 2019.
• Web of Science Citations: 1

Abstract

Antioxidants protect cells from the injuries caused by oxidative reactive species, especially under biotic and abiotic stressful conditions. Each kind of free radical presents different oxidative sites and, therefore, different assays could be done to better evaluate the antioxidant capacity of a compound. Little is known about secondary metabolites from Annona coriacea Mart. (Annonaceae), especially related to their antioxidant capacity. Eleven flavonols were isolated from leaves: Quercetin-3-O-gentiobioside (1), Quercetin-3-O-robinobioside (2), Rutin (3), Hyperin (4), Isoquercitrin (5), Biorobin (6), Nicotiflorin (7), Keioside (8), Narcissin (9), Cacticin (10) and Isorhamnetin-3-O-glucoside (11). The isolated compounds and their commercial aglycones were evaluated in DPPH, ABTS(+center dot) radical scavenging, ferric reducing antioxidant power (FRAP) and oxygen reduction antioxidant capacity (ORAC). Two patterns of response were observed. In the first, found in DPPH and FRAP assays, quercetin and its derivatives (1-5) showed higher antioxidant activity than kaempferol (6-7) and isorhamnetin derivatives (8-11). On the other hand, in the second pattern, observed in ABTS(+center dot) and ORAC assays, isorhamnetin showed higher or similar activities than quercetin derivatives. These differences were attributed to the B-ring substitution pattern of flavonols and to the distinct mechanisms of antioxidant activity in each assay. Different assays must be performed to determine the mechanisms of antiradicalar action of a compound. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/07543-0 - Pesticide activities of acetogenins of native species of Annonaceae from Brazilian Savanna
• Grantee: Déborah Yara Alves Cursino dos Santos
• Support Opportunities: Regular Research Grants