| Full text | |
| Author(s): |
Kawakita, Erika R. H.
[1]
;
Re, Ana Carolina S.
[1]
;
Peixoto, Maria Paula G.
[2]
;
Ferreira, Maira P.
[2]
;
Ricomini-Filho, Antonio P.
[3]
;
Freitas, Osvaldo
[2]
;
Aires, Carolina P.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Piracicaba, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Molecules; v. 24, n. 9 MAY 1 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
(1) Background: The effectiveness of chitosan to improve the action of antimicrobial compounds against planktonic bacteria and young biofilms has been widely investigated in Dentistry, where the biofilm lifecycle is a determining factor for the success of antibacterial treatment. In the present study, mature Streptococcus mutans biofilms were treated with chitosan dispersion (CD) or chitosan microparticles (CM). (2) Methods: CD at 0.25% and 1% were characterized by texture analysis, while CD at 2% was spray-dried to form CM, which were characterized with respect to particle size distribution, zeta potential, and morphology. After determining the minimum inhibitory and bactericidal concentrations, S. mutans biofilms were grown on glass slides exposed 8x/day to 10% sucrose and 2x/day to CD or CM at 0.25% and 1%. Biofilm viability and acidogenicity were determined, using appropriate control groups for each experiment. (3) Results: CD had high viscosity and CM were spherical, with narrow size distribution and positive zeta potential. CM affected bacterial viability and acidogenicity in mature S. mutans biofilms more strongly than CD, especially at 1%. (4) Conclusions: Both chitosan forms exerted antimicrobial effect against mature S. mutans biofilms. CM at 1% can reduce bacterial viability and acidogenicity more effectively than CD at 1%, and thereby be more effective to control the growth of mature biofilms in vitro. (AU) | |
| FAPESP's process: | 15/17712-9 - Strategies for sustained antimicrobial delivery to biofilms |
| Grantee: | Carolina Patrícia Aires Garbellini |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/04363-6 - Gel and microparticles of chitosan for buccal biofilm control |
| Grantee: | Erika Reiko Hashimoto Kawakita |
| Support Opportunities: | Scholarships in Brazil - Master |