| Full text | |
| Author(s): |
Godoy-Veiga, Milena
[1]
;
Slotta, Franziska
[2]
;
Alecio, Paula Christiani
[1]
;
Ceccantini, Gregorio
[1]
;
Buckeridge, Marcos Silveira
[1]
;
Locosselli, Giuliano Maselli
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biosci, Dept Bot, Rua Matao 277, BR-05508090 Sao Paulo - Brazil
[2] Free Univ Berlin, Inst Geol Sci, Dept Earth Sci, Malteserstr 74-100, D-12249 Berlin - Germany
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | DENDROCHRONOLOGIA; v. 55, p. 33-42, JUN 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
The great diversity of wood anatomical features found in trees worldwide results in a broad variety of growthring boundary types that are not always easy to recognize, especially in tropical woods. However, the presence of clearly visible limits between tree rings is essential for any tree-ring studies. Here, we propose the use of autofluorescence of wood in order to enhance tree-ring visualization. The multispectral light emitted from the fluorescence stereomicroscope can be filtered in specific wavelengths to improve the visualization of wood anatomical features. To evaluate the effectiveness of this technique, we compared visualization under natural light, GFP (green fluorescent protein) filter, RFP (red fluorescent protein) filter and UV filter. We tested this technique with a set of 38 tree species with different types of growth-ring boundaries. Although results are species-specific, fluorescence has been shown to improve the visualization of growth-ring boundaries by enhancing the contrast among cell types. It may highlight fibrous zones (e.g. Cavanillesia arborea, Aspidosperma polyneuron), different porosity patterns (e.g. Myracrodruon urundeuva), secretory canals (e.g. Copaifera langsdorffu), and parenchyma bands (e.g. Tipuana tipu). Fluorescence allows the visualization of growth-ring boundaries in species that were previously described as having indistinct growth rings under natural light. For species with clear tree-ring boundaries such as Cedrela fissilis and Hymenaea courbaril, this approach aids the identification of false rings. In addition to the visualization of growth-ring boundaries, autofluorescence may be useful for other qualitative and quantitative analyses of wood anatomy, such as wood identification and automated measurements of anatomical features. Scientists struggling with tree-ring counting and cross-dating due to difficult tree-ring visualization may find fluorescence useful. It may also aid to identify new species suitable for tree-ring studies. (AU) | |
| FAPESP's process: | 13/21728-2 - The use of modern autopsy techniques to investigate human diseases (MODAU) |
| Grantee: | Paulo Hilário Nascimento Saldiva |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/25511-3 - Climate and pollution reconstruction based on urban tree-rings growth records |
| Grantee: | Giuliano Maselli Locosselli |
| Support Opportunities: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 17/50085-3 - PIRE: climate research education in the Americas using tree-ring speleothem examples (PIRE-CREATE) |
| Grantee: | Francisco William da Cruz Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 08/57908-6 - National Institute of Science and Technology of Bioethanol |
| Grantee: | Marcos Silveira Buckeridge |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |