Approaches for the isolation and identification of hydrophilic, light-sensitive, volatile and minor natural products

Author(s)

Berlinck, Roberto G. S. ^[1] ; Monteiro, Afif F. ^[1] ; Bertonha, Ariane F. ^[1] ; Bernardi, Darlon I. ^[1] ; Gubiani, Juliana R. ^[1] ; Slivinski, Juliano ^[1] ; Michaliski, Lamonielli F. ^[1] ; Tonon, Luciane A. C. ^[1] ; Venancio, Victor A. ^[1] ; Freire, Vitor F. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Review article
• Source: NATURAL PRODUCT REPORTS; v. 36, n. 7, p. 981-1004, JUL 1 2019.
• Web of Science Citations: 2

Abstract

Covering: up to 2019 The discovery of new bioactive natural products gained momentum during the last few decades, resulting from instrumentation advances, from the expansion of genome mining and regulation, as well as by exploration of untapped biological sources. However, water-soluble, volatile, minor and photosensitive natural products are yet poorly known. This review discusses the literature reporting the isolation strategies for some of these metabolites. Analysis of minor metabolites at sub-milligram level are also presented, since analytical instrumentation enabling structure assignment in minute quantities is now routine. Major trends related to natural products discovery are discussed, under the light of further developments in biodiscovery. (AU)

• FAPESP's process: 16/21341-9 - Hydrophilic and bioactive secondary metabolites produced by filamentous fungi
• Grantee: Darlon Irineu Bernardi
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 17/06014-4 - New secondary metabolites of endophytic and marine organisms as proteasome inhibitors: a validated target against cancer
• Grantee: Juliana Regina Gubiani
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/14375-4 - Omics tools applied to the discovery of tetrodotoxin producing organisms and biosynthetic genes
• Grantee: Luciane Alessandra Chimetto Tonon
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/11949-0 - Cytotoxic secondary metabolites from two strains of endophytic fungi from anthurium loefgrenii
• Grantee: Victor Alves Venâncio
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/50228-8 - Biodiversity components, and their metabolic characters, of Brazilian Islands: an integrated approach
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 17/25100-9 - Discovery of bioactive secondary metabolites produced by microbial strains from isolated environments
• Grantee: Lamonielli Fagá Michaliski
• Support Opportunities: Scholarships in Brazil - Master