Effects of glutamine and alanine supplementation on muscle fatigue parameters of rats submitted to resistance training

Author(s)

Coqueiro, Audrey Yule ^[1] ; Raizel, Raquel ^[1] ; Bonvini, Andrea ^[1] ; Rogero, Marcelo Macedo ^{[2, 3]} ; Tirapegui, Julio ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo - Brazil

^[2] Sao Paulo Res Fdn, Res Innovat & Disseminat Ctr, Food Res Ctr FoRC, CEPID FAPESP, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Publ Hlth, Dept Nutr, Sao Paulo - Brazil

• Document type: Journal article
• Source: NUTRITION; v. 65, p. 131-137, SEP 2019.
• Web of Science Citations: 1

Abstract

Objective: Although glutamine and alanine have properties that could delay fatigue, recent evidence showed that these amino acids impaired central fatigue markers. Nevertheless, the effect of this intervention on muscle fatigue is unknown. The aim of this study was to investigate the effects of glutamine and alanine supplementation on muscle fatigue parameters in rats submitted to resistance training (RT). Methods: Wistar rats were distributed into the following groups: sedentary (SED), exercised (CON), exercised and supplemented with alanine (ALA), glutamine and alanine in their free form (G+A) or L-alanyl-L-glutamine (DIP). Trained groups underwent a ladder-climbing exercise for 8 wk. In the last 3 wk of RT, supplementations were offered in water with a 4% concentration. Results: G+A and DIP supplementation increased the muscle content of glutamine and glutamate. DIP administration increased glycogen and lactate dehydrogenase (LDH) concentrations in muscle, whereas ALA and G +A supplementation reduced plasma LDH and creatine kinase levels. All trained groups presented higher levels of muscle glutathione (GSH) than SED. There was no difference between groups in lactate, xanthine, hypoxanthine, thiobarbituric acid reactive substances, 8-isoprostane and GSH in plasma; adenosine monophosphate deaminase, citrate synthase and monocarboxylate transporters 1 and 4 in muscle; and glycogen and GSH in the liver. Moreover, physical performance did not differ between groups. Conclusion: Glutamine and alanine supplementation improved muscle fatigue markers without affecting exercise performance. (C) 2018 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 16/04910-0 - Effect of chronic supplementation with L-glutamine and L-alanine, in free form or as dipeptide, on parameters associated with fatigue in rats subjected to resistance training.
• Grantee: Julio Orlando Tirapegui Toledo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/22789-3 - Effect of chronic oral supplementation with L-glutamine and L-alanine, in their free forms or as dipeptide, on parameters associated with fatigue in rats submitted to resistance exercise
• Grantee: Audrey Yule Coqueiro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)