Systemic expression of genes related to inflammation and lipid metabolism in patients with dyslipidemia, type 2 diabetes mellitus and chronic periodontitis

Author(s)

Nepomuceno, Rafael ^{[1, 2]} ; Vallerini, Bruna de E. ^[1] ; da Silva, Romerito L. ^[2] ; Corbi, Sarnia C. T. ^{[1, 2]} ; Bastos, Alliny de S. ^[2] ; dos Santos, Raquel A. ^[3] ; Takahashi, Catarina S. ^{[4, 5]} ; Orrico, Silvana Regina P. ^[2] ; Scarel-Caminaga, Raquel M. ^[1]

Affiliation

^[1] UNESP Sao Paulo State Univ, Sch Dent Araraquara, Dept Morphol, CP 331, BR-14801903 Araraquara, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP - Brazil

^[3] Univ Franca, Postgrad Program Sci, BR-14404600 Franca, SP - Brazil

^[4] Univ Sao Paulo, FFCLRP, Fac Med Ribeirao Preto, Dept Genet, BR-14040900 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, FFCLRP, Dept Biol, BR-14040900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: DIABETES & METABOLIC SYNDROME-CLINICAL RESEARCH & REVIEWS; v. 13, n. 4, p. 2715-2722, JUL-AUG 2019.
• Web of Science Citations: 1

Abstract

Inflammatory diseases, as periodontal disease (PD), has been associated with disturbance of lipid and glycemic metabolisms, as demonstrated by the increasing of PD patients with type 2 diabetes mellitus (T2D) and/or dyslipidemia comorbidities. We aimed to investigate the expression of inflammation and lipid metabolism genes, and correlations among clinical and biochemical characteristics in normoglycemic or T2D patients with dyslipidemia and PD, in comparison with healthy individuals. Five groups of 30 individuals each (150 patients) were formed based upon T2D, dyslipidemic and periodontal status. Blood analyses of lipid and glycemic profiles were carried out, and the gene expression was assessed by RT-qPCR. The systemic expression of 1L6, TNFA and LEP genes were significantly higher in T2D, dyslipidemia and PD patients, while the PECAM1 gene showed the opposite. Higher RETN levels were found in patients with T2D independently of their glycemic control status. There were positive correlations between: TNFA, LEP and RETN with worse periodontal parameters; 1L6, TNFA, ADIPOR1, LEP and RETN with waist-to-hip ratio; glycemic parameters with RETN; total cholesterol and triglycerides with LEP expression. We conclude that pro-inflammatory cytokines were related with worse lipid, glycemic and periodontal parameters, reinforcing that a hyper-inflammatory status connects systemic and oral inflammatory diseases. (C) 2019 Diabetes India. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/19699-7 - Gene expression of IL6 and TNFA in individuals with type 2 Diabetes Mellitus, Dyslipidemia and Periodontal Disease
• Grantee: Bruna de Freitas Vallerini
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 07/08362-8 - Correlation between lipid peroxidation and inflammatory markers profile in diabetes mellitus type 2 patients with chronic periodontal disease
• Grantee: Silvana Regina Perez Orrico
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/16233-9 - Evaluation on the effect of the Diabetes Mellitus decompensation on the gene expression of the immune system and DNA damage in chronic periodontitis patients.
• Grantee: Sâmia Cruz Tfaile Corbi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/10882-2 - Gene expression in individuals with type 2 Diabetes mellitus , dyslipidemia and periodontal disease: evaluation by microarray
• Grantee: Raquel Mantuaneli Scarel Caminaga
• Support Opportunities: Regular Research Grants