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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium

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Author(s):
Andrade, Gabriella Mamede [1] ; Bomfim, Monalisa Medrado [1] ; del Collado, Maite [1] ; Meirelles, Flavio Vieira [1] ; Perecin, Felipe [1] ; da Silveira, Juliano Coelho [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Sao Paulo, Pirassununga - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Molecular Reproduction and Development; v. 86, n. 8, p. 1067-1080, AUG 2019.
Web of Science Citations: 0
Abstract

The biotechnology for in vitro embryo production is becoming increasingly popular, being applied to humans and domestic animals. Embryo development can be achieved with either 20% or 5% oxygen tension. The extracellular vesicles (EVs) are secreted by different cell types and carry bioactive materials. Our objective was to determine the secretion pattern and micro RNA (miRNA) contents of EVs released in the bovine embryo culture environment-embryo and cumulus cell monolayer-on Days 3 and 7 of in vitro culture under two different oxygen tensions: High (20%) and low (5%). The EVs were isolated from the medium and analyzed to determine size, concentration, and miRNA levels. EVs concentration in low oxygen tension increased on Day 3 and decreased on Day 7. Additionally, altered EV miRNAs derived from the embryo-cumulus culture medium were predicted to regulate survival and proliferation-related pathways on Days 3 and 7. Moreover, miR-210 levels decreased in EVs isolated from the culture medium under high oxygen tension suggesting that this miRNA can be used as a marker for normoxia since it is associated with low oxygen tension. In summary, this study provides knowledge of the oxygen tension effects on EVs release and content, and potentially, on cell-to-cell communication during in vitro bovine embryo production. (AU)

FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
Grantee:Marcelo Fábio Gouveia Nogueira
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/21034-3 - Intra and intercellular routes during foliculogenesis and in vivo and in vitro oocyte maturation
Grantee:Felipe Perecin
Support type: Regular Research Grants
FAPESP's process: 15/21829-9 - Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10
Grantee:Juliano Coelho da Silveira
Support type: Multi-user Equipment Program
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 14/21042-6 - Effects of oxidative stress during bovine in vitro embryo production on miR199a and its target genes ERBB2 and ERBB3
Grantee:Monalisa Medrado Bomfim
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC