Interface-driven Sr-morin complexation at Langmuir monolayers for bioactive coating design

Author(s)

Cruz, M. A. E. ^[1] ; Soares, M. P. R. ^[2] ; Pazin, W. ^{[3, 4]} ; Ito, A. S. ^[3] ; Fukada, S. Y. ^[2] ; Ciancaglini, P. ^[1] ; Ramos, A. P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Fis, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo - Brazil

^[4] Univ Estadual Paulista, Dept Fis, Fac Ciencias & Tecnol, Sao Paulo - Brazil

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 181, p. 856-863, SEP 1 2019.
• Web of Science Citations: 0

Abstract

Flavonoid-metal complexes are widely studied because of their interesting luminescent behavior and biological activity. Despite the extensive exploration of flavonoid-metal coordination processes in solution, the formation of complexes using the flavonoid molecule inserted in a lipid membrane has been little investigated. This effect could provide important insight into the biological activity of flavonoids at lipid membranes and could represent an attractive strategy to design supramolecular structures. Here, we studied the complexation between Sr2+ and morin inserted in an octadecylphosphonic acid (OPA) Langmuir monolayer. This is a relevant system due to the synergism imposed by the association of the Sr2+ ability to control bone formation/resorption with the morin antioxidative effect. Morin incorporation into the OPA monolayers and further Sr2+ complexation were monitored by surface pressure isotherms. Electronic absorption spectroscopy and fluorescence techniques showed Srmorin complexation both in solution and at the air-liquid interface. Although morin complexation has been described to occur only at basic pH, the specific thermodynamic properties at the air-liquid interface drove metal complexation. LB films were deposited on Ti surfaces, and the resulting OPA/Sr-morin coatings exhibited high surface free energy and increase on its polar component. This optimized surface feature supported further serum protein adsorption and osteoblast growth and differentiation, indicating that these lipid-based coatings are promising for bioactive coating design. This study paves the way for the use of this lipid-based coating in the design of implants for faster osteointegration. Moreover, flavonoid-metal complexation at membranes could also help to shed light on the biological role played by flavonoids. (AU)

• FAPESP's process: 15/08774-0 - Modification of Ti surfaces by Langmuir-Blodgett hybrid films containing biominerals and strontium ranelate to promote the controled Sr2+ release
• Grantee: Marcos Antonio Eufrásio Cruz
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 17/08892-9 - Bioactive surfaces designed from Langmuir-Blodgett Films and Biominerals
• Grantee: Ana Paula Ramos
• Support type: Regular Research Grants
• FAPESP's process: 16/21236-0 - Extracellular matrix vesicles (MVs) mimetic systems to study the regulation of the biomineralization process: proteoliposomes containing NPP1 and Annexin V
• Grantee: Pietro Ciancaglini
• Support type: Regular Research Grants