Study of the interaction of the glycopeptide vancomycin with cell membrane models formed by lipid Langmuir films

• Grant number: 19/04402-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): July 01, 2019
• Effective date (End): January 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Heung Jin Alves Santana
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

In this project, we intend to study the action of vancomycin with Langmuir films of phospholipids, which serve as simple models of cell membranes. The phospholipids to be used will be dipalmitoyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidyl ethanolamine (DPPE), co-mixed with the bioactive. The interaction of vancomycin with the phospholipid monolayers will be evaluated by means of pressure and surface potential measurements, Brewster angle microscopy, infrared spectroscopy, and surface rheology. The results will be evaluated from the perspective of possible biological implications, inferred from the effects caused by the bioactive on the artificial membranes.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, HEUNG JIN ALVES; CASELI, LUCIANO. A bactericide peptide changing the static and dilatational surface elasticity properties of zwitterionic lipids at the air-water interface: Relationship with the thermodynamic, structural and morphological properties. Biophysical Chemistry, v. 277, OCT 2021. (19/03239-0, 18/22214-6, 19/04402-2)