Study of cationic lipid and water soluble polymers polymers interaction in solution and in vesicular molecular films as biomimetic model systems

• Grant number: 11/07414-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): June 01, 2011
• Effective date (End): May 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Eloi da Silva Feitosa
• Grantee: Renata Danielle Adati
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

Intensive investigation of synthetic cationic vesicles properties of alkyl dimethylammonium, DnDAB, n = 10-18 is of represent a great importance for basic and applied science, because it is a model for biological membrane, and especially for drug delivery and cell transfection in genetherapy. In this project we propose a systematic study of physicochemical properties related with DnDAB vesicles formation and interaction with water soluble polymers (polyethylene glycol or polyacrilic acid, for example polymers. It will be varied the concentration of DnDAB and the relative amount of additive in order to obtain the properties of the vesicles and the vesicle-additive complexes, such as the hydrodynamic radius, the gel-liquid crystalline, pre and post-transitions states temperature, and the surface potentials (zeta potentials). The dynamic interactions between vesicles and polymers will be monitored trough Langmuir-Blodgett techniques, which is largely employed in biological context due high sensibility detection in interfacial systems that simulate cell membrane interacting with different species. The monolayer transfer will be performed by adequate immobilized groups at the substrate; the physicochemical properties related with organized systems will be investigated. The interdisciplinarity of this project recognizes the relevance of academic studies to applications in different research areas.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FEITOSA, ELOI; ADATI, RENATA D.; HANSSON, PER; MALMSTEN, MARTIN. Thermal and Structural Behavior of Dioctadecyldimethylammonium Bromide Dispersions Studied by Differential Scanning Calorimetry and X-Ray Scattering. PLoS One, v. 7, n. 9, SEP 6 2012. (11/00796-4, 11/07414-0)

NORBERTO OLIVEIRA, ANA CRISTINA; MARTENS, THOMAS FRANS; RAEMDONCK, KOEN; ADATI, RENATA DANIELLE; FEITOSA, ELOI; BOTELHO, CLAUDIA; GOMES, ANDREIA CASTRO; BRAECKMANS, KEVIN; DIAS REAL OLIVEIRA, MARIA ELISABETE CUNHA. Dioctadecyldimethylammonium:Monoolein Nanocarriers for Efficient in Vitro Gene Silencing. ACS APPLIED MATERIALS & INTERFACES, v. 6, n. 9, p. 6977-6989, MAY 14 2014. (11/03566-0, 11/07414-0)

ADATI, RENATA D.; FEITOSA, ELOI. The assembly of dialkyldimethylammonium bromide cationic lipids as vesicles or monolayers in presence of poly(ethylene glycol). Thermochimica Acta, v. 613, p. 71-76, AUG 10 2015. (11/03566-0, 11/07414-0)

FEITOSA, ELOI; LEMOS, MONIQUE; ADATI, RENATA D.. Mixed Cationic Surfactant Vesicles in (Dioctadecyldimethylammonium Bromide)/NaCl and (Dioctadecyldimethylammonium Chloride)/NaBr Aqueous Dispersions. JOURNAL OF SURFACTANTS AND DETERGENTS, v. 22, n. 5, JULY 2019. (11/07414-0)

FEITOSA, ELOSI; ADATI, RENATA D.; ALVES, FERNANDA R.. Thermal and phase behavior of didodecyldimethylammonium bromide aqueous dispersions. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 480, p. 253-259, SEP 5 2015. (11/03566-0, 11/07414-0)