Improving wave-induced motion bioreactor performance for human mesenchymal stromal cell expansion

Author(s)

da Silva, Juliana de Sa ^[1] ; Mizukami, Amanda ^[2] ; Gonzalez Gil, V, Liseth ; de Camposa, Jessica Valeria ^{[3, 4]} ; Assis, Odilio B. G. ^[3] ; Covas, Dimas Tadeu ^[2] ; Swiechb, Kamilla ^{[2, 5]} ; Torres Suazo, Claudio Alberto ^[4]

Affiliation

^[1] Univ Fed Sao Carlos, Chem Engn Dept, Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Reg Blood Ctr Ribeirao Preto, Ctr Cell Based Therapy CTC, Ribeirao Preto, SP - Brazil

^[3] Embrapa Instrumentat, Sao Carlos, SP - Brazil

^[4] Gonzalez Gil, Liseth, V, Univ Fed Sao Carlos, Chem Engn Dept, Sao Carlos, SP - Brazil

^[5] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Cafe Av W-N, BR-14040903 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Process Biochemistry; v. 84, p. 143-152, SEP 2019.
• Web of Science Citations: 0

Abstract

Mesenchymal Stromal Cells (MSCs) are considered a viable option for the treatment of several diseases in different medical areas. MSCs are present in small proportions in tissues, thus making an in vitro expansion necessary in accordance with the cGMP. For this purpose, the disposable Wave-Induced Motion Bioreactor (WIMB) is a promising alternative for MSC expansion in a safe and low shear environment. However, wave motion leads to the formation of aggregate deposits (microcarriers and cells) on the Cellbag plastic, impairing the MSC proliferation. In this study, a modification in the Cellbag design was proposed and tested in order to overcome this limitation. Human Umbilical Cord Matrix derived Mesenchymal Stromal Cells (hUCM-MSCs) were expanded in WIMB using Cultispher-S microcarriers and alpha-MEM medium. Two approaches were evaluated for hUCM-MSCs culture in the new Cellbag design: 1) adhesion phase in spinner and expansion in the WIMB (Expansion Factors: 5.8-8.0 fold) and 2) adhesion and expansion phases in the WIMB (Expansion Factors: 11.6-25.6 fold), both with absence of the most damaging particle deposits. These results showed that the use of WIMB with the Cellbag design modification have good potential for hUCM-MSCs expansion, while maintaining cell quality attributes. (AU)

• FAPESP's process: 12/23228-4 - In vitro expansion of mesenchymal stromal cells and secretome characterization: therapeutic and biotechnological applications
• Grantee: Amanda Mizukami Martins
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC