Efficient hydrolysis of wine and grape juice anthocyanins by Malbranchea pulchella beta-glucosidase immobilized on MANAE-agarose and ConA-Sepharose supports

Author(s)

Oliveira Monteiro, Lummy Maria ^[1] ; Pereira, Marita Gimenez ^[2] ; Vici, Ana Claudia ^[2] ; Heinen, Paulo Ricardo ^[1] ; Buckeridge, Marcos S. ^[3] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Bandeirantes Av 3-900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Bandeirantes Av 3-900, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Matao St 277, BR-05508090 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 136, p. 1133-1141, SEP 1 2019.
• Web of Science Citations: 0

Abstract

beta-glucosidases (BGLs) hydrolyze short-chain cellulooligosaccharides. Some BGLs can hydrolyze anthocyanins and be applied in the clarification process of food industries, especially grape juice and wine. Enzyme immobilization is a valuable tool to increase enzyme stabilization. In this work, Malbranchea pulchella BGL was immobilized on Monoaminoethyl-N-ethyl-agarose ionic support, MANAE-agarose, and Concanavalin A-Sepharose affinity support, Con-A-Sepharose. The formed biocatalysts, denominated BLG-MANAE and BLG-ConA, were applied in the grape juice and red wine clarification. BGL-MANAE and BGL-ConA hyperactivated M. pulchella BGL 10- and 3-fold, respectively. Both biocatalysts showed at least 70% activity at pH range 2-11, until 24 h incubation. BGL-MANAE and BGL-ConA showed activity of 60% and 100%, respectively, at 50 degrees C, up to 24 h. Both biocatalysts were efficiently reused 20-fold. They were stable in the presence of up to 0.1 M glucose for 24 h incubation, and with 5%, 10% and 15% ethanol kept up to 70% activity. BGL-MANAE biocatalyst was 11% and 25% more efficient than BGL-ConA in clarification of concentrate and diluted wines, respectively. Likewise, BGL-MANAE biocatalysts were 14% and 33% more efficient than the BGL-ConA in clarification of diluted and concentrated juices, respectively. Therefore, the BGL-MANAE biocatalyst was especially effective in red wine and grape juice clarification. (C) 2019 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support Opportunities: Research Projects - Thematic Grants