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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei

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Campos Antonieto, Amanda Cristina [1] ; Vieira Nogueira, Karoline Maria [1] ; de Paula, Renato Graciano [1] ; Nora, Lufsa Czamanski [1] ; Anzolini Cassiano, Murilo Henrique [1] ; Guazzaroni, Maria-Eugenia [2] ; Almeida, Fausto [1] ; da Silva, Thiago Aparecido [1] ; Ries, Laure Nicolas Annick [3] ; de Assis, Leandro Jose [3] ; Goldman, Gustavo Henrique [3, 4] ; Silva, Roberto Nascimento [1] ; Silva-Rocha, Rafael [1]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, FMRP, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, FFCLRP, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, FCFRP, Ribeirao Preto, SP - Brazil
[4] Tech Univ Munich, Inst Adv Study, Garching - Germany
Total Affiliations: 4
Document type: Journal article
Source: MSYSTEMS; v. 4, n. 4 JUL-AUG 2019.
Web of Science Citations: 1
Abstract

Filamentous fungi are remarkable producers of enzymes dedicated to the degradation of sugar polymers found in the plant cell wall. Here, we integrated transcriptomic data to identify novel transcription factors (TFs) related to the control of gene expression of lignocellulosic hydrolases in Trichoderma reesei and Aspergillus nidulans. Using various sets of differentially expressed genes, we identified some putative cis-regulatory elements that were related to known binding sites for Saccharomyces cerevisiae TFs. Comparative genomics allowed the identification of six transcriptional factors in filamentous fungi that have corresponding S. cerevisiae homologs. Additionally, a knockout strain of T. reesei lacking one of these TFs (S. cerevisiae AZF1 homolog) displayed strong reductions in the levels of expression of several cellulase-encoding genes in response to both Avicel and sugarcane bagasse, revealing a new player in the complex regulatory network operating in filamentous fungi during plant biomass degradation. Finally, RNA sequencing (RNA-seq) analysis showed the scope of the AZF1 homologue in regulating a number of processes in T. reesei, and chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) provided evidence for the direct interaction of this TF in the promoter regions of ce17a, ce145a, and swo. Therefore, we identified here a novel TF which plays a positive effect in the expression of cellulase-encoding genes in T. reesei. IMPORTANCE In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. in vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms. (AU)

FAPESP's process: 16/03322-7 - Role of galectin-3 in the Cryptococcus neoformans infection
Grantee:Fausto Bruno dos Reis Almeida
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 18/03766-8 - Uncovering the regulatory network of Trichoderma reesei: characterization of the RME1 homologue binding motif and identification of new regulators involved in the biomass degradation
Grantee:Amanda Cristina Campos Antoniêto
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/23233-9 - Characterization of calcium-dependent signaling pathway in the secretion and cellulase expression by Trichoderma reesei (Hypocrea jecorina
Grantee:Renato Graciano de Paula
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/03763-3 - Unveiling the mechanisms of gene regulation at the single cell level in Trichoderma reesei
Grantee:Luísa Czamanski Nora
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries
Grantee:María Eugenia Guazzaroni
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters
Grantee:Rafael Silva Rocha
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 17/04217-5 - Development of a computational tool for searching and strength prediction of bacterial promoters for applications in synthetic biology
Grantee:Murilo Henrique Anzolini Cassiano
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/09553-8 - Characterization of new sugar transporters involved in the regulation of lignocellulosic biomass degradation of in Trichoderma reesei
Grantee:Karoline Maria Vieira Nogueira
Support type: Scholarships in Brazil - Doctorate