Influence of protease inhibitors on the degradation of sound, sclerotic and caries-affected demineralized dentin

Author(s)

Oliveira-Reis, B. ^[1] ; Maluly-Proni, A. T. ^[2] ; Fagundes, T. C. ^[1] ; Vasconcelos, G. ^[3] ; Bresciani, E. ^[4] ; Prakki, A. ^[5] ; dos Santos, P. H. ^[2]

Affiliation

^[1] Sao Paulo State Univ, Aracatuba Sch Dent, Dept Restorat Dent, 1193 Jose Bonifacio St, BR-16015050 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ, Aracatuba Sch Dent, Dept Dent Mat & Prosthodont, 1193 Jose Bonifacio St, BR-16015050 Aracatuba, SP - Brazil

^[3] Inst Adv Studies Sao Jose Campos IEAv, Photon Div Lasers, Dept Aerosp Sci & Technol, 01 Putim, BR-12228001 Sao Jose Dos Campos, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sci & Technol Inst Sao Jose Campos, Dept Restorat Dent, 777 Eng Francisco Jose Longo Ave, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[5] Univ Toronto, Fac Dent, Dept Restorat Dent, 124 Edward St, Toronto, ON M5G 1G6 - Canada

• Document type: Journal article
• Source: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 97, p. 1-6, SEP 2019.
• Web of Science Citations: 1

Abstract

The aim of this study was to evaluate influence of protease inhibitors on degradation of sound, sclerotic and caries-affected dentin. Thirty-nine molars were used, thirteen for each dentin condition. Three slices were obtained from each tooth, each one immersed in the following different solutions for 1 h: 1) artificial saliva; 2) 2% chlorhexidine; 3) 0.5% epigallocatechin-gallate (EGCG). After immersion, samples were subjected to enzymatic degradation challenge (collagenase from Clostridium histolyticum). Nanohardness (HIT) and elastic modulus (Er) were measured before and after enzymatic challenge, as well as the ultimate tensile strength (UTS). Results of UTS, HIT and Er tests were submitted to repeated measurements ANOVA and Tukey post-test (alpha = 0.05). Higher values of UTS were found for sound dentin in control (40.30 +/- 21.38 MPa) and 0.5% EGCG (30.05 +/- 19.67 MPa) groups. Before degradation, higher values of HIT (0.237 +/- 0.062 GPa) and Er (5.58 +/- 1.75 GPa) were found for 0.5% EGCG group in caries-affected dentin. After degradation, 2% chlorhexidine group had higher values of HIT and Er for sound (0.134 +/- 0.020 GPa and 3.57 +/- 0.40 GPa) and sclerotic (0.201 +/- 0.048 GPa and 4.30 +/- 0.56 GPa) dentin. The 2% chlorhexidine showed best effect increasing HIT and Er, mainly for sclerotic dentin, before/after enzymatic degradation. The 0.5% EGCG showed better effect on HIT and Er in caries-affected dentin, before enzymatic degradation. (AU)

• FAPESP's process: 15/10566-7 - Effect of protease inhibitors on collagen degradation potential from sound, sclerotic and caries-affected dentin
• Grantee: Bruna de Oliveira Reis
• Support Opportunities: Scholarships in Brazil - Master