Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The importance of Toll-like receptor 4 during experimental Sporothrix brasiliensis infection

Full text
Rossato, Luana [1] ; dos Santos, Suelen Silvana [1] ; Ferreira, Lucas Goncalves [1] ; de Almeida, Sandro Rogerio [1]
Total Authors: 4
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Medical Mycology; v. 57, n. 4, p. 489-495, JUN 2019.
Web of Science Citations: 0

Here we investigated the importance of Toll-like receptor 4 (TLR-4) in innate immune response to Sporothrix brasiliensis, a virulent fungus of Sporothrix spp. In vitro assays, using C57Bl/6 (wild type {[}WT]) bone marrow-derived macrophages (BMDMs), and TLR-4 knockout (TLR-4(-/-)) showed that the absence of TLR-4 resulted in impaired phagocytosis and lower levels of tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, and nitric oxide. In vivo assays were also performed, and the mice (WT and TLR-4(-/-)) were intraperitoneally infected with S. brasiliensis yeast ATCC MyA-4831 and euthanized on days 7, 14, and 28 postinfection, with the following parameters evaluated: fungal burden in liver, spleen, kidney, and brain, and the production of cytokines interferon gamma (IFN-alpha), TNF-alpha, IL-2, IL-4, IL-6, and IL-10. The results demonstrate the macrophages dependency on TLR-4 for inflammatory activation and in the absence of TLR-4 during experimental S. brasiliensis infection enhanced dissemination occurred after 14 and 28 days. These data show that TLR-4 signals are important for the recognition of S. brasiliensis by macrophages, and their absence promotes the persistence of the infection. (AU)

FAPESP's process: 13/19213-4 - Study of interaction between macrophages opposite species Sporothrix brasiliensis
Grantee:Luana Rossato
Support type: Scholarships in Brazil - Doctorate