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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The importance of Toll-like receptor 4 during experimental Sporothrix brasiliensis infection

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Author(s):
Rossato, Luana [1] ; dos Santos, Suelen Silvana [1] ; Ferreira, Lucas Goncalves [1] ; de Almeida, Sandro Rogerio [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Medical Mycology; v. 57, n. 4, p. 489-495, JUN 2019.
Web of Science Citations: 0
Abstract

Here we investigated the importance of Toll-like receptor 4 (TLR-4) in innate immune response to Sporothrix brasiliensis, a virulent fungus of Sporothrix spp. In vitro assays, using C57Bl/6 (wild type {[}WT]) bone marrow-derived macrophages (BMDMs), and TLR-4 knockout (TLR-4(-/-)) showed that the absence of TLR-4 resulted in impaired phagocytosis and lower levels of tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, and nitric oxide. In vivo assays were also performed, and the mice (WT and TLR-4(-/-)) were intraperitoneally infected with S. brasiliensis yeast ATCC MyA-4831 and euthanized on days 7, 14, and 28 postinfection, with the following parameters evaluated: fungal burden in liver, spleen, kidney, and brain, and the production of cytokines interferon gamma (IFN-alpha), TNF-alpha, IL-2, IL-4, IL-6, and IL-10. The results demonstrate the macrophages dependency on TLR-4 for inflammatory activation and in the absence of TLR-4 during experimental S. brasiliensis infection enhanced dissemination occurred after 14 and 28 days. These data show that TLR-4 signals are important for the recognition of S. brasiliensis by macrophages, and their absence promotes the persistence of the infection. (AU)

FAPESP's process: 13/19213-4 - Study of interaction between macrophages opposite species Sporothrix brasiliensis
Grantee:Luana Rossato
Support type: Scholarships in Brazil - Doctorate