Titanium alloys: in vitro biological analyzes on biofilm formation, biocompatibility, cell differentiation to induce bone formation, and immunological response

Author(s)

Reis Mello, Daphne de Camargo ^[1] ; de Oliveira, Jonatas Rafael ^[1] ; Alves Cairo, Carlos Alberto ^[2] ; de Brito Ramos, Lais Siebra ^[1] ; da Cruz Vegian, Mariana Raquel ^[1] ; de Vasconcellos, Luis Gustavo Oliveira ^[3] ; de Oliveira, Felipe Eduardo ^[4] ; de Oliveira, Luciane Dias ^[1] ; Reis de Vasconcellos, Luana Marotta ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Air & Space Inst CTA, Div Mat, Praca Marechal do Ar Eduardo Gomes 14, BR-12904000 Sao Jose Dos Campos, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Mat & Dent Prosthodont, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[4] Univ Ctr Brazcubas, Brazcubas Fac Dent, Av Francisco Rodrigues Filho 1233, BR-08773380 Mogi Das Cruzes, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 30, n. 9 SEP 2019.
• Web of Science Citations: 0

Abstract

Biological effects of titanium (Ti) alloys were analyzed on biofilms of Candida albicans, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans, and Streptococcus sanguinis, as well as on osteoblast-like cells (MG63) and murine macrophages (RAW 264.7). Standard samples composed of aluminum and vanadium (Ti-6Al-4V), and sample containing niobium (Ti-35Nb) and zirconium (Ti-13Nb-13Zr) were analyzed. Monomicrobial biofilms were formed on the Ti alloys. MG63 cells were grown with the alloys and the biocompatibility (MTT), total protein (TP) level, alkaline phosphatase (ALP) activity, and mineralization nodules (MN) formation were verified. Levels of interleukins (IL-1 beta and IL-17), tumor necrosis factor alpha (TNF-alpha), and oxide nitric (NO) were checked, from RAW 264.7 cells supernatants. Data were statically analyzed by one-way analysis of variance (ANOVA) and Tukey's test, or T-test (P <= 0.05). Concerning the biofilm formation, Ti-13Nb-13Zr alloy showed the best inhibitory effect on E. faecalis, P. aeruginosa, and S. aureus. And, it also acted similarly to the Ti-6Al-4V alloy on C. albicans and Streptococcus spp. Both alloys were biocompatible and similar to the Ti-6Al-4V alloy. Additionally, Ti-13Nb-13Zr alloy was more effective for cell differentiation, as observed in the assays of ALP and MN. Regarding the stimulation for release of IL-1 beta and TNF-alpha, Ti-35Nb and Ti-13Nb-13Zr alloys inhibited similarly the synthesis of these molecules. However, both alloys stimulated the production of IL-17. Additionally, all Ti alloys showed the same effect for NO generation. Thus, Ti-13Nb-13Zr alloy was the most effective for inhibition of biofilm formation, cell differentiation, and stimulation for release of immune mediators. (AU)

• FAPESP's process: 15/01258-7 - In vitro evaluation of metabolic cell activity about different titanium alloys samples aiming for biomedical applications
• Grantee: Laís Siebra de Brito Ramos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/20311-8 - In vivo comparison of biocompatibility of porous surface samples made with different titanium alloys
• Grantee: Luana Marotta Reis de Vasconcellos
• Support Opportunities: Regular Research Grants