Young and elderly oral squamous cell carcinoma patients present similar angiogenic profile and predominance of M2 macrophages: Comparative immunohistochemical study

Author(s)

Teixeira, Lucas R. ^[1] ; Almeida, Luciana Y. ^[2] ; Silva, Rodrigo N. ^[3] ; Mesquita, Ana T. M. ^[4] ; Colturato, Carla B. N. ^[1] ; Silveira, Heitor A. ^[3] ; Duarte, Andressa ^[1] ; Ribeiro-Silva, Alfredo ^[1] ; Leon, Jorge E. ^[3]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, Dept Pathol & Forens Med, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, Dept Clin Med, Hematol Div, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Dent Sch FORP, Dept Stomatol Publ Oral Hlth & Forens Dent, Oral Pathol, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[4] Fed Univ Jequitinhonha & Mucuri Valleys, Dept Dent Clin Stomatol, Diamantina, MG - Brazil

• Document type: Journal article
• Source: HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK; v. 41, n. 12, p. 4111-4120, DEC 2019.
• Web of Science Citations: 0

Abstract

Background M2 macrophages are often detected in oral squamous cell carcinoma (OSCC), which, influenced by hypoxic conditions, appear to have high angiogenesis-inducing capacity. However, the effects of immunosenescence on tumor-associated macrophages (TAMs) and angiogenesis in OSCC are unknown. Methods Fifty-seven OSCCs were divided into 3 groups (I: 65 years {[}n = 20]). Immunohistochemistry for CD68 and CD163 (TAMs), and CD34 and D2-40 for microvessel density (MVD), microvessel area (MVA), and total vascular area (TVA) were performed. Results All groups showed similar clinicopathological and immunohistochemical findings. Similar CD68 and CD163 expression, confirmed a M2 phenotype. MVD, MVA, and TVA were similar, however, with significant predominance of blood vessels. No significant correlation between macrophage and angiogenic markers was observed. Conclusions A similar TAM and angiogenesis profile suggests the participation of other mechanisms, instead immunosenescence, in young and elderly OSCC patients. (AU)

• FAPESP's process: 18/12734-2 - Comparative immunohistochemical characterization of dendritic cell subgroups and viral oncogenesis in oral and oropharyngeal squamous cell carcinoma
• Grantee: Heitor Albergoni da Silveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/11419-0 - Clinicopathological correlation of the degree of differentiation, tetraspanin expression, viral agents and cell proliferation in a large sample of squamous cell carcinoma of the head and neck region.
• Grantee: Jorge Esquiche León
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/02502-4 - M1 and M2 macrophages and its relation with angiogenesis in oral squamous cell carcinomas affecting young and elderly patients
• Grantee: Lucas Ribeiro Teixeira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/02713-2 - Effects of erlotinib and gefitinib therapy in combination with all-trans retinoic acid and arsenic trioxide in acute promyelocytic leukemia: in vitro, ex vivo and murine experimental model studies.
• Grantee: Luciana Yamamoto de Almeida
• Support Opportunities: Scholarships in Brazil - Post-Doctoral