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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Montelukast, Leukotriene Inhibitor, Reduces LPS-Induced Acute Lung Inflammation and Human Neutrophil Activation

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Davino-Chiovatto, Jaime Eduardo [1] ; Oliveira-Junior, Manoel Carneiro [2] ; MacKenzie, BreAnne [2] ; Santos-Dias, Alana [2] ; Almeida-Oliveira, Ana Roberta [2] ; Jonco Aquino-Junior, Jefferson Comin [2] ; Brito, Aurileia Aparecida [1] ; Rigonato-Oliveira, Nicole Cristine [1] ; Damaceno-Rodrigues, Nilsa Regina [3] ; Ligeiro Oliveira, Ana Paula [1] ; Silva, Alessandro Pereira [4] ; Consolim-Colombo, Fernanda Marciano [1] ; Aimbire, Flavio [5] ; Castro-Faria-Neto, Hugo Caire [6] ; Vieira, Rodolfo Paula [2, 7, 8, 9]
Total Authors: 15
Affiliation:
[1] Nove de Julho Univ UNINOVE, Sao Paulo, SP - Brazil
[2] Brazilian Inst Teaching & Res Pulm & Exercise Imm, Sao Jose Dos Campos, SP - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Pathol LIM 59, Sao Paulo, SP - Brazil
[4] Univ Mogi das Cruzes, Postgrad Program Biomed Engn, Mogi Das Cruzes, SP - Brazil
[5] Fed Univ Sao Paulo UNIFESP, Sao Jose Dos Campos, SP - Brazil
[6] Osvaldo Cruz Fdn, Osvaldo Cruz Inst IOC, Lab Immunopharmacol, Rio De Janeiro, RJ - Brazil
[7] Fed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, Santos, SP - Brazil
[8] Univ Brasil, Postgrad Program Bioengn & Biomed Engn, Sao Paulo, SP - Brazil
[9] Anhembi Morumbi Univ, Sch Med, Ave Deputado Benedito Matarazzo 4050, Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 9
Document type: Journal article
Source: ARCHIVOS DE BRONCONEUMOLOGIA; v. 55, n. 11, p. 573-580, NOV 2019.
Web of Science Citations: 1
Abstract

Objectives: Some pro-inflammatory lipids derived from 1 lipooxygenase enzyme are potent neutrophil chemoattractant, a cell centrally involved in acute respiratory distress syndrome (ARDS); a syndrome lacking effective treatment. Considering the beneficial effects of the leukotriene receptor inhibitor, montelukast, on other lung diseases, whether montelukast attenuates inflammation in a mouse model of ARDS, and whether it reduces LPS stimulated activation of human neutrophils was investigated. Methods: Thirty-five C57Bl/6 mice were distributed into control (PBS) + 24h, LPS + 24 h (10 mu g/mouse), control +48 h, LPS +48 h, and LPS 48 h + Montelukast (10 mg/kg). In addition, human neutrophils were incubated with LPS (1 mu g/mL) and treated with montelukast (10 mu M). Results: Oral-tracheal administration of montelukast significantly attenuated total cells (P < .05), macrophages (P< .05), neutrophils (P< .01), lymphocytes (P< .001) and total protein levels in BAL (P < .05), as well as IL-6 (P < .05), CXCL1/KC (P < .05), IL-17 (P< .05) and TNF-alpha (P< .05). Furthermore, montelukast reduced neutrophils (P<.001), lymphocytes (P < .01) and macrophages (P< .01) in the lung parenchyma. In addition, montelukast restored BAL VEGF levels (P<.05). LTB4 receptor expression (P< .001) as well as NF-kappa B (P<.001), a downstream target of LPS, were also reduced in lung parenchymal leukocytes. Furthermore, montelukast reduced IL-8 (P < .001) production by LPS-treated human neutrophils. Conclusion: In conclusion, montelukast efficiently attenuated both LPS-induced lung inflammation in a mouse model of ARDS and in LPS challenged human neutrophils. (C) 2019 SEPAR. Published by Elsevier Espatia, S.L.U. All rights reserved. (AU)

FAPESP's process: 14/23196-0 - The role of purinergic signalling and of SOCS-JAK-STAT signalling in the anti-inflammatory effects of aerobic training in asthmatic individuals.
Grantee:Breanne Mackenzie
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/12755-9 - Effects of aerobic physical training in obese asthmatic mice
Grantee:Jefferson Comin Jonco Aquino Junior
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/14604-8 - Role of purinergic signaling in the antiinflammatory effects of aerobic training in a model of asthma induced by house dust mite
Grantee:Manoel Carneiro de Oliveira Junior
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/07500-1 - Role of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic exercise in an experimental model of asthma
Grantee:Ana Roberta Almeida Comin
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
Grantee:Rodolfo de Paula Vieira
Support type: Research Grants - Young Investigators Grants