Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain

Author(s)

Brenelli, Livia B. ^{[1, 2]} ; Persinoti, Gabriela F. ^[1] ; Franco Cairo, Joao Paulo L. ^{[3, 4]} ; Liberato, Marcelo V. ^[3] ; Goncalves, Thiago Augusto ^{[3, 4]} ; Otero, Igor V. R. ^[5] ; Mainardi, Pedro H. ^[5] ; Felby, Claus ^[2] ; Sette, Lara D. ^[5] ; Squina, Fabio M. ^[3]

Affiliation

^[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil

^[2] Univ Copenhagen, Fac Sci, Dept Geosci & Nat Resource Management, Frederiksberg C - Denmark

^[3] Univ Sorocaba UNISO, Programa Proc Tecnol & Ambientais, Sorocaba - Brazil

^[4] Univ Campinas UNICAMP, Inst Biol, Dept Bioquim & Biol Tecidual, Campinas, SP - Brazil

^[5] Univ Estadual Paulista UNESP, Inst Biociencias, Rio Claro - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 9, NOV 26 2019.
• Web of Science Citations: 0

Abstract

The repertoire of redox-active enzymes produced by the marine fungus Peniophora sp. CBMAI 1063, a laccase hyper-producer strain, was characterized by omics analyses. The genome revealed 309 Carbohydrate-Active Enzymes (CAZymes) genes, including 48 predicted genes related to the modification and degradation of lignin, whith 303 being transcribed under cultivation in optimized saline conditions for laccase production. The secretome confirmed that the fungus can produce a versatile ligninolytic enzyme cocktail. It secretes 56 CAZymes, including 11 oxidative enzymes classified as members of auxiliary activity families (AAs), comprising two laccases, Pnh\_Lac1 and Pnh\_Lac2, the first is the major secretory protein of the fungi. The Pnh\_Lac1-mediator system was able to promote the depolymerization of lignin fragments and polymeric lignin removal from pretreated sugarcane bagasse, confirming viability of this fungus enzymatic system for lignocellulose-based bioproducts applications. (AU)

• FAPESP's process: 13/03061-0 - Characterization and antioxidant capacity of fractions of lignin extracted from sugarcane bagasse
• Grantee: Lívia Beatriz Brenelli de Paiva
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/19486-0 - Marine and Antarctic biotechnology: microbial enzymes and their applications
• Grantee: Lara Durães Sette
• Support type: Regular Research Grants
• FAPESP's process: 15/07008-2 - Understanding the complex structure of lignin from sugarcane bagasse and the impact on the antioxidant capacity and its biochemical conversion into value added products
• Grantee: Lívia Beatriz Brenelli de Paiva
• Support type: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 14/04105-4 - Structural and functional characterization of new cellulases, focusing in the relation between catalytic domains and CBMs
• Grantee: Marcelo Vizoná Liberato
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 16/09950-0 - Functional, structural characterizations and biotechnological application of lytic polysaccharide monooxygenases from the lower termite Coptotermes gestroi
• Grantee: João Paulo Lourenço Franco Cairo
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 15/06971-3 - Investigation of oxidative mechanisms involved on lignocellulosic biomass breakdown by the lower termite Coptotermes gestroi
• Grantee: João Paulo Lourenço Franco Cairo
• Support type: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
• Grantee: Fábio Márcio Squina
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 17/16089-1 - Production of lignin chains, metagenomic screening and biocatalytic conversion of lignin to ferulic acid
• Grantee: Thiago Augusto Gonçalves
• Support type: Scholarships in Brazil - Post-Doctorate