Ionic liquid associated with ultrasonic-assisted extraction: A new approach to obtain carotenoids from orange peel

Author(s)

Murador, Daniella C. ^[1] ; Braga, Anna Rafaela C. ^{[1, 2]} ; Martins, Paula L. G. ^[3] ; Mercadante, Adriana Z. ^[4] ; de Rosso, Veridiana V. ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, UNIFESP, Dept Biosci, Rua Silva Jardim 136, BR-11015020 Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, UNIFESP, Dept Chem Engn, Rua Sao Nicolau 201, BR-09972270 Diadema, SP - Brazil

^[3] Fed Inst Sao Paulo, Av Clara Gianotti de Souza 5180, BR-11900000 Registro, SP - Brazil

^[4] Univ Estadual Campinas, UNICAMP, Fac Food Engn, Dept Food Sci, Rua Monteiro Lobato 70, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: Food Research International; v. 126, DEC 2019.
• Web of Science Citations: 1

Abstract

The aim of this study was to develop a new method for carotenoid extraction from orange peel, using ionic liquid (IL) to replace conventional organic solvents, assisted by ultrasound. Four different IL were tested: 1-butyl-3-methylimidazolium chloride ({[}BMIM]{[}Cl]), 1-n-butyl-3-methylimidazolium hexafluorophosphate ({[}BMIM]{[}PF6]), 1-n-butyl-3-methylimidazolium tetrafluoroborate ({[}BMIM]{[}BF4]), and 1-hexyl-3-methylimidazolium chloride ({[}HMIM]{[}Cl]). Response surface methodology was applied in order to optimize the carotenoid extraction conditions, and Amberlite XAD-7HP resin was used to separate the carotenoids from the IL, allowing their recovery. Determination of carotenoids was carried out by high-performance liquid chromatography coupled to photodiode array and mass spectrometry detectors (HPLC-DAD-MSn). Thermal stability at different temperatures (60 degrees C and 90 degrees C) and peroxyl radical scavenging activity of the carotenoid extracts obtained with acetone and IL were evaluated. {[}BMIM] {[}Cl] was the most effective IL, leading to a total carotenoid content of 32.08 +/- 2.05 mu g/g, while 7.88 +/- 0.59 mu g/g of dry matter was obtained by acetone extraction. IL and carotenoid recoveries using XAD-7HP resin were in the range of 59.5-63.8% and 52.2-58.7%, respectively. A carotenoid extract was successfully obtained with IL, finally isolated just by using ethanol, besides being more stable and presenting higher antioxidant activity than that obtained with acetone. (AU)

• FAPESP's process: 15/26789-5 - Ionic Liquids As Green Extractors for Achievement of Carotenoids from Orange Peel
• Grantee: Daniella Carisa Murador
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/18910-1 - Ionic Liquids as Green Extractors for Achievement of Carotenoids: Study of Bioavailability and Antioxidant Activit
• Grantee: Veridiana Vera de Rosso
• Support Opportunities: Regular Research Grants