DNA methylation and one-carbon metabolism related nutrients and polymorphisms: analysis after mandatory flour fortification with folic acid

Author(s)

Steluti, Josiane ^[1] ; Palchetti, Cecilia Zanin ^[1] ; Miranda, Andreia Machado ^[1] ; Fisberg, Regina Mara ^[1] ; Marchioni, Dirce Maria ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-01246904 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: BRITISH JOURNAL OF NUTRITION; v. 123, n. 1, p. 23-29, JAN 14 2020.
• Web of Science Citations: 0

Abstract

There is a growing research interest in determining whether changes in the global status of DNA methylation are related to the environment, in particular, to one-carbon metabolism. So, our aim was to investigate the effect of dietary methyl-group donor intake (methionine, folate, choline, betaine, vitamins B-2, B-6 and B-12), biomarkers (total folate, unmetabolised folic acid (FA), 5-methyltetrahydrofolate, homocysteine, vitamins B-6 and B-12 concentrations) and genetic variants (polymorphisms involved in one-carbon metabolism) on global DNA methylation in a population exposed to mandatory flour fortification with FA. A cross-sectional study of health and living conditions was conducted among a representative sample of residents in Sao Paulo, Brazil. The mean of global DNA methylation was lower in young people than in adults and the elderly (P = 0 center dot 049). No differences between genotypes of polymorphism and global DNA methylation mean were identified. We observed that the increase in betaine intake led to an absolute change in percentage of DNA methylation (beta = 0 center dot 0005, P = 0 center dot 024) using multiple regression. Betaine intake alone was associated with an absolute change in percentage of global DNA methylation. The study did not find an association between global DNA methylation and folate status even in a population exposed to mandatory flour fortification with FA. (AU)

• FAPESP's process: 11/19788-1 - Assessment of folate status after mandatory folic acid fortification and health consequences: population-based study in the City of São Paulo
• Grantee: Dirce Maria Lobo Marchioni
• Support type: Regular Research Grants
• FAPESP's process: 12/05505-0 - Standardization of methodology and analysis of unmetabolized folic acid after mandatory fortification with folic acid: population-based study in São Paulo City
• Grantee: Josiane Steluti
• Support type: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 10/19899-5 - Dietary intake and genetic variants of folate and its relation to folate, unmetabolized folic acid and homocysteine blood concentrations after mandoty folic acid fortification: population based study - ISA-Capital.
• Grantee: Josiane Steluti
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/12196-2 - UNMETABOLIZED FOLIC ACID AND ITS ASSOCIATIONS WITH GLOBAL DNA METHYLATION, OXIDATIVE STRESS, INFLAMMATORY MARKERS AND METABOLIC SIGNATURE: ANALYSIS AFTER MANDATORY FORTIFICATION OF FLOUR WITH FOLIC
• Grantee: Josiane Steluti
• Support type: Scholarships in Brazil - Post-Doctorate