Detection and Whole-Genome Analysis of a High-Risk Clone of Klebsiella pneumoniae ST340/CG258 Producing CTX-M-15 in a Companion Animal

Author(s)

Valencia-Bacca, Juan ^[1] ; Silva, Meire M. ^[2] ; Cerdeira, Louise ^[3] ; Esposito, Fernanda ^[3] ; Cardoso, Brenda ^[4] ; Munoz, Maria E. ^[3] ; Jimenez-Villegas, Tatiana ^[1] ; Cardenas-Arias, Adriana ^[4] ; Pessoa, Danielle A. N. ^[5] ; Lincopan, Nilton ^{[4, 3]}

Affiliation

^[1] Univ Sao Paulo, Dept Prevent Vet Med & Anim Hlth, Sao Paulo, SP - Brazil

^[2] Centro Univ Joao Pessoa, Vet Med Acad Unit, Joao Pessoa, Paraiba - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo, SP - Brazil

^[5] Univ Fed Campina Grande, Sch Vet Med, Campina Grande, Paraiba - Brazil

• Document type: Journal article
• Source: MICROBIAL DRUG RESISTANCE; v. 26, n. 6 DEC 2019.
• Web of Science Citations: 0

Abstract

The emergence and dissemination of high-risk clones of Klebsiella pneumoniae producing extended-spectrum beta-lactamases (ESBLs) in animal infections is a critical issue. We report the detection and genomic features of a multidrug-resistant (MDR) ESBL (CTX-M-15)-producing K. pneumoniae infecting a domestic cat. Whole-genome sequencing analysis identified the international ST340 (clonal group CG258), and genes and mutations conferring resistance to beta-lactams, aminoglycosides, macrolides, phenicols, fosfomycin, sulfonamides, tetracycline, trimethoprim, and fluoroquinolones. In addition, the presence of genes encoding resistance to disinfectant and heavy metals hazardous to humans was also confirmed. The MDR profile exhibited by the strain contributed to treatment failure and death of the companion animal. Therefore, active surveillance of critical priority lineages of K. pneumoniae should not only focus on human infections but also on veterinary infections. (AU)

• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants