Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan

Author(s)

Mohsin, Mashkoor ^[1] ; Azam, Mariya ^[1] ; Rahman, Sajjad Ur ^[1] ; Esposito, Fernanda ^[2] ; Sellera, Fabio P. ^[3] ; Monte, Daniel F. ^[4] ; Cerdeira, Louise ^[1] ; Lincopan, Nilton ^{[2, 5]}

Affiliation

^[1] Univ Agr Faisalabad, Inst Microbiol, Jhang Rd, Faisalabad 38000 - Pakistan

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Vet Med & Anim Scid, Dept Internal Med, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Food Res Ctr, Dept Food & Expt Nutr, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Av Prof Lineu Prestes 1374, BR-05508000 Sao Paulo - Brazil

• Document type: Journal article
• Source: PATHOGENS AND DISEASE; v. 77, n. 7 OCT 2019.
• Web of Science Citations: 0

Abstract

The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and bla(CARB-2), besides cma (colicin M) and astA {[}heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health. (AU)

• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/15578-4 - Virulome and pathogenicity of carbapenem- and polymyxin-resistant priority pathogens
• Grantee: Fernanda Ribeiro dos Santos Esposito
• Support Opportunities: Scholarships in Brazil - Doctorate