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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lipoatrophy-Associated Insulin Resistance and Hepatic Steatosis are Attenuated by Intake of Diet Rich in Omega 3 Fatty Acids

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Moreira, Rafael J. [1] ; Castro, Erique [1] ; Oliveira, Tiago E. [1] ; Belchior, Thiago [1] ; Peixoto, Albert S. [1] ; Chaves-Filho, Adriano B. [1] ; Moreno, Mayara F. [1] ; Lima, Janayna D. [1] ; Yoshinaga, Marcos [2] ; Miyamoto, Sayuri [2] ; Morais, Mychel R. P. T. [3] ; Zorn, Telma M. T. [3] ; Cogliati, Bruno [4] ; Iwai, Leo K. [5] ; Palmisano, Giuseppe [6] ; Cabral, Fernanda J. [7] ; Festuccia, William [1]
Total Authors: 17
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Cell Biol, BR-05508000 Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Pathol, Sch Vet Med & Anim Sci, BR-05508010 Sao Paulo - Brazil
[5] Butantan Inst, Ctr Toxins Immune Response & Cell Signaling LETA, Special Lab Appl Toxicol, BR-05503400 Sao Paulo - Brazil
[6] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, BR-05508000 Sao Paulo - Brazil
[7] Univ Estadual Campinas, Dept Anim Biol, BR-13083862 Campinas - Brazil
Total Affiliations: 7
Document type: Journal article
Source: MOLECULAR NUTRITION & FOOD RESEARCH; v. 64, n. 7 FEB 2020.
Web of Science Citations: 1
Abstract

Scope Glucose homeostasis and progression of nonalcoholic fatty liver disease (NAFLD) and hepatomegaly in severe lipoatrophic mice and their modulation by intake of a diet rich in omega 3 (n-3) fatty acids (HFO) are evaluated. Methods and results Severe lipoatrophic mice induced by PPAR-gamma deletion exclusively in adipocytes (A-PPAR gamma KO) and littermate controls (A-PPAR gamma WT) are evaluated for glucose homeostasis and liver mass, proteomics, lipidomics, inflammation, and fibrosis. Lipoatrophic mice are heavier than controls, severely glucose intolerant, and hyperinsulinemic, and develop NAFLD characterized by increased liver glycogen, triacylglycerol, and diacylglycerol contents, mitotic index, apoptosis, inflammation, steatosis score, fibrosis, and fatty acid synthase (FAS) content and activity. Lipoatrophic mice also display liver enrichment with monounsaturated in detriment of polyunsaturated fatty acids including n-3 fatty acids, and increased content of cardiolipin, a tetracyl phospholipid exclusively found at the mitochondria inner membrane. Administration of a high-fat diet rich in n-3 fatty acids (HFO) to lipoatrophic mice enriches liver with n-3 fatty acids, reduces hepatic steatosis, FAS content and activity, apoptosis, inflammation, and improves glucose homeostasis. Conclusion Diet enrichment with n-3 fatty acids improves glucose homeostasis and reduces liver steatosis and inflammation without affecting hepatomegaly in severe lipoatrophic mice. (AU)

FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 16/04000-3 - Pharmacology of oligopeptidases and intracellular peptides
Grantee:Emer Suavinho Ferro
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/17943-6 - Proteomic and peptidomic analysis of snake venom effect on cancer cell lines and mouse brain tissues
Grantee:Leo Kei Iwai
Support Opportunities: Regular Research Grants
FAPESP's process: 15/19530-5 - Involvement of the nutrient sensor mTOR in the development of obesity associated chronic metabolic diseases
Grantee:William Tadeu Lara Festuccia
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications
Grantee:Giuseppe Palmisano
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications
Grantee:Giuseppe Palmisano
Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC